LOCUS       sequence-151670-        1102 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of single-cysteine variant of mature phosphate binding 
            protein, PstS, as scaffold for phosphate biosensor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1102)
  AUTHORS   Solscheid C, Kunzelmann S, Davis CT, Hunter JL, Nofer A, Webb MR
  TITLE     Development of a Reagentless Biosensor for Inorganic Phosphate, 
            Applicable over a Wide Concentration Range.
  JOURNAL   Biochemistry. 2015 Aug 18;54(32):5054-62. doi: 
            10.1021/acs.biochem.5b00449. Epub 2015 Aug 4.
  PUBMED    26199994
REFERENCE   2  (bases 1 to 1102)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acs.biochem.5b00449"; journalName: "Biochemistry"; date: 
            "2015-08-18- 18"; volume: "54"; issue: "32"; pages: "5054-62"
COMMENT     Sequence Label: pET22b_PstS_1
FEATURES             Location/Qualifiers
     source          1..1102
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(31..48)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             1034..1056
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    1071..1095
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 ctttcgggct ttgttagcag ccggatctca gtggtggtgg tggtggtgct cgagtcatta
       61 gtacagcggc ttaccgctac tgtctttaat attggtcttc cacgcagcgc gaacctgttc
      121 aactacacta tccggcaggc tggcgtaatc caggtcgttc gcctgtttag ccccggtttt
      181 gtacgcccag tcgaagaatt tcagcacttc tgtgccttgt tctggtttct tctgatcttt
      241 gtggatcaga atgaacgtgg tagaggtaat aggccatgca tcttcgcctt tctggttggt
      301 cagatcctga gcgaaggttt tgctccagtc tgcacctttt gctgcattag cgaagttttc
      361 ttcggtcgga ctaaccggtt taccatcagc ggagatcagt ttggtgtacg ccaggttgtt
      421 ctgcttcgcg taacaatatt caacataacc aattgcaccc ggcagacgct gaacgaacgc
      481 ggcgataccg tcgttacctt taccgcccag accgatcggc cattttacgg tagagccagt
      541 accaacgttg tttttccact cttcgttcac tttcgccagg tagctggtga agacgaagga
      601 agtcccggag ccatctgcgc ggcgtactac agcaatgttt tgtgaaggca gtttcagacc
      661 cggattcagt ttggcgatgg cttcatcatc ccacttcttg attttgccca ggtagatgtc
      721 gccgagggtt ttaccatcca gcaccagttc gccagacttc agccctggaa tgttaaccgc
      781 cagcaccacg ccgccaatca cggtcgggaa ctggaacaga ccttcctgag ccagtttttc
      841 gtcagacagc ggcgcgtcag aggcaccaaa atcaacggta ttagcgataa tctgttttac
      901 gccaccggaa gaaccgatac cctggtagtt aactttatta ccggtttctt tctggtaagt
      961 gtcagcccat ttggcataca ccggcgcagg gaaggttgca cctgcacctg tcaggcttgc
     1021 ttccatatgt atatctcctt cttaaagtta aacaaaatta tttctagagg ggaattgtta
     1081 tccgctcaca attcccctat ag
//
