LOCUS       sequence-151811-         806 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3rd gen lentiviral V5-Luciferase expression vector, PGK promoter, 
            Puro.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 806)
  AUTHORS   Campeau E, Ruhl VE, Rodier F, Smith CL, Rahmberg BL, Fuss JO, 
            Campisi J, Yaswen P, Cooper PK, Kaufman PD.
  TITLE     A versatile viral system for expression and depletion of proteins in
            mammalian cells.
  JOURNAL   PLoS One. 2009 Aug 6;4(8):e6529.
  PUBMED    19657394
REFERENCE   2  (bases 1 to 806)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2009-08-6"; volume: "4(8)"; pages: "e6529"
COMMENT     Sequence Label: pLenti PGK V5-LUC Puro (w543-1)
FEATURES             Location/Qualifiers
     source          1..806
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    89..113
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             125..166
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     regulatory      236..245
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(308..326)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
ORIGIN
        1 cctcgttgac cgaatcaccg acctctctcc ccagggggat ccaccggttc gtcgactagt
       61 ccagtgtggt ggaattctgc agatatcaac aagtttgtac aaaaaagcag gctccaccat
      121 ggaaggtaag cctatcccta accctctcct cggtctcgat tctacgggat ccatgggaac
      181 caattcagtc cctaggcttt tgcaaaaagc ttggcattcc ggtactgttg gtaaagccac
      241 catggaagac gccaaaaaca taaagaaagg cccggcgcca ttctatccgc tggaagatgg
      301 aaccgctgga gagcaactgc ataaggctat gaagagatac gccctggttc ctggaacaat
      361 tgcttttaca gatgcacata tcgaggtgga catcacttac gctgagtact tcgaaatgtc
      421 cgttcggttg gcagaagcta tgaaacgata tgggctgaat acaaatcaca gaatcgtcgt
      481 atgcagtgaa aactctcttc aattctttat gccggtgttg ggcgcgttat ttatcggagt
      541 tgcagttgcg cccgcgaacg acatttataa tgaacgtgaa ttgctcaaca gtatgggcat
      601 ttcgcagcct accgtggtgt tcgtttccaa aaaggggttg caaaaaattt tgaacgtgca
      661 aaaaaagctc ccaatcatcc aaaaaattat tatcatggat tctaaaacgg attaccaggg
      721 atttcagtcg atgtacacgt tcgtcacatc tcatctacct cccggtttta atgaatacga
      781 ttttgtgcca gagtccttcg ataggg
//
