LOCUS       sequence-151930-        9028 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  E coli Genetic Modification.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9028)
  AUTHORS   Yang J, Sun B, Huang H, Jiang Y, Diao L, Chen B, Xu C, Wang X, Liu 
            J, Jiang W, Yang S
  TITLE     High-Efficiency Scarless Genetic Modification in Escherichia coli by
            Using Lambda Red Recombination and I-SceI Cleavage.
  JOURNAL   Appl Environ Microbiol. 2014 Jul 1;80(13):3826-3834. Epub 2014 Apr 
            18.
  PUBMED    24747889
REFERENCE   2  (bases 1 to 9028)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl 
            Environ Microbiol."; date: "2014-07-1"; volume: "80(13)"; pages: 
            "3826-3834. Epub 2014 Apr 18"
COMMENT     Sequence Label: pREDKI
FEATURES             Location/Qualifiers
     source          1..9028
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(19..897)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     promoter        924..1208
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     1131..1150
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     RBS             1235..1243
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             1252..1668
                     /codon_start=1
                     /gene="gam"
                     /product="inhibitor of the host RecBCD nuclease in the 
                     lambda Red system"
                     /label=Gam
                     /note="protects the double-stranded DNA substrate for 
                     recombination"
                     /translation="MDINTETEIKQKHSLTPFPVFLISPAFRGRYFHSYFRSSAMNAYY
                     IQDRLEAQSWARHYQQLAREEKEAELADDMEKGLPQHLFESLCIDHLQRHGASKKSITR
                     AFDDDVEFQERMAEHIRYMVETIAHHQVDIDSEV"
     CDS             1674..2459
                     /codon_start=1
                     /gene="bet"
                     /product="single-stranded DNA binding recombinase in the 
                     lambda Red system"
                     /label=Beta
                     /note="mediates pairing of a single-stranded DNA with a 
                     complementary target"
                     /translation="MSTALATLAGKLAERVGMDSVDPQELITTLRQTAFKGDASDAQFI
                     ALLIVANQYGLNPWTKEIYAFPDKQNGIVPVVGVDGWSRIINENQQFDGMDFEQDNESC
                     TCRIYRKDRNHPICVTEWMDECRREPFKTREGREITGPWQSHPKRMLRHKAMIQCARLA
                     FGFAGIYDKDEAERIVENTAYTAERQPERDITPVNDETMQEINTLLIALDKTWDDDLLP
                     LCSQIFRRDIRASSELTQAEAVKALGFLKQKAAEQKVAA"
     CDS             2456..3136
                     /codon_start=1
                     /gene="exo"
                     /product="5' to 3' double-stranded DNA exonuclease in the 
                     lambda Red system"
                     /label=Exo
                     /note="creates 3' single-stranded DNA tails"
                     /translation="MTPDIILQRTGIDVRAVEQGDDAWHKLRLGVITASEVHNVIAKPR
                     SGKKWPDMKMSYFHTLLAEVCTGVAPEVNAKALAWGKQYENDARTLFEFTSGVNVTESP
                     IIYRDESMRTACSPDGLCSDGNGLELKCPFTSRDFMKFRLGGFEAIKSAYMAQVQYSMW
                     VTRKNAWYFANYDPRMKREGLHYVVIERDEKYMASFDEIVPEFIEKMDEALAEIGFVFG
                     EQWR"
     terminator      3137..3381
                     /label=lambda tL3 terminator
                     /note="transcription terminator tL3 from phage lambda"
     CDS             complement(3414..4262)
                     /codon_start=1
                     /gene="E. coli rhaR"
                     /product="transcriptional activator of rhaR and rhaS"
                     /label=rhaR
                     /note="needed for rhamnose-dependent activation of the rhaB
                     promoter"
                     /translation="MAHQLKLLKDDFFASDQQAVAVADRYPQDVFAEHTHDFCELVIVW
                     RGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQNIIYCPERLKLNLDWQGAI
                     PGFNASAGQPHWRLGSMGMAQARQVIGQLEHESSQHVPFANEMAELLFGQLVMLLNRHR
                     YTSDSLPPTSSETLLDKLITRLAASLKSPFALDKFCDEASCSERVLRQQFRQQTGMTIN
                     QYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMTPSQWRHLNSQK
                     D"
     CDS             complement(4336..5172)
                     /codon_start=1
                     /gene="E. coli rhaS"
                     /product="positive regulator of the rhaB promoter
                     "
                     /label=rhaS
                     /note="activated by L-rhamnose"
                     /translation="MTVLHSVDFFPSGNASVAIEPRLPQADFPEHHHDFHEIVIVEHGT
                     GIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLLPQ
                     ELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKSSLQ
                     ENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQRYLNR
                     LRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGFLQ"
     promoter        5318..5436
                     /label=rhaB promoter
                     /note="promoter of the E. coli rhaBAD operon, conferring 
                     tight induction with L-rhamnose and repression with 
                     D-glucose in the presence of RhaR and RhaS (Giacalone et 
                     al., 2006)"
     CDS             complement(6205..7155)
                     /codon_start=1
                     /gene="rep101"
                     /product="RepA protein needed for replication with the 
                     pSC101 origin"
                     /label=Rep101
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLRNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
     rep_origin      7203..7425
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     primer_bind     complement(8180..8199)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             8191..9006
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             8191..9006
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(8264..8283)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
ORIGIN
        1 aaatggttta aacatttatt atgacaactt gacggctaca tcattcactt tttcttcaca
       61 accggcacgg aactcgctcg ggctggcccc ggtgcatttt ttaaataccc gcgagaaata
      121 gagttgatcg tcaaaaccaa cattgcgacc gacggtggcg ataggcatcc gggtggtgct
      181 caaaagcagc ttcgcctggc tgatacgttg gtcctcgcgc cagcttaaga cgctaatccc
      241 taactgctgg cggaaaagat gtgacagacg cgacggcgac aagcaaacat gctgtgcgac
      301 gctggcgata tcaaaattgc tgtctgccag gtgatcgctg atgtactgac aagcctcgcg
      361 tacccgatta tccatcggtg gatggagcga ctcgttaatc gcttccatgc gccgcagtaa
      421 caattgctca agcagattta tcgccagcag ctccgaatag cgcccttccc cttgcccggc
      481 gttaatgatt tgcccaaaca ggtcgctgaa atgcggctgg tgcgcttcat ccgggcgaaa
      541 gaaccccgta ttggcaaata ttgacggcca gttaagccat tcatgccagt aggcgcgcgg
      601 acgaaagtaa acccactggt gataccattc gcgagcctcc ggatgacgac cgtagtgatg
      661 aatctctcct ggcgggaaca gcaaaatatc acccggtcgg caaacaaatt ctcgtccctg
      721 atttttcacc accccctgac cgcgaatggt gagattgaga atataacctt tcattcccag
      781 cggtcggtcg ataaaaaaat cgagataacc gttggcctca atcggcgtta aacccgccac
      841 cagatgggca ttaaacgagt atcccggcag caggggatca ttttgcgctt cagccatact
      901 tttcatactc ccgccattca gagaagaaac caattgtcca tattgcatca gacattgccg
      961 tcactgcgtc ttttactggc tcttctcgct aaccaaaccg gtaaccccgc ttattaaaag
     1021 cattctgtaa caaagcggga ccaaagccat gacaaaaacg cgtaacaaaa gtgtctataa
     1081 tcacggcaga aaagtccaca ttgattattt gcacggcgtc acactttgct atgccatagc
     1141 atttttatcc ataagattag cggatcctac ctgacgcttt ttatcgcaac tctctactgt
     1201 ttctccatac ccgttttttt gggaattcga gctctaagga ggttataaaa aatggatatt
     1261 aatactgaaa ctgagatcaa gcaaaagcat tcactaaccc cctttcctgt tttcctaatc
     1321 agcccggcat ttcgcgggcg atattttcac agctatttca ggagttcagc catgaacgct
     1381 tattacattc aggatcgtct tgaggctcag agctgggcgc gtcactacca gcagctcgcc
     1441 cgtgaagaga aagaggcaga actggcagac gacatggaaa aaggcctgcc ccagcacctg
     1501 tttgaatcgc tatgcatcga tcatttgcaa cgccacgggg ccagcaaaaa atccattacc
     1561 cgtgcgtttg atgacgatgt tgagtttcag gagcgcatgg cagaacacat ccggtacatg
     1621 gttgaaacca ttgctcacca ccaggttgat attgattcag aggtataaaa cgaatgagta
     1681 ctgcactcgc aacgctggct gggaagctgg ctgaacgtgt cggcatggat tctgtcgacc
     1741 cacaggaact gatcaccact cttcgccaga cggcatttaa aggtgatgcc agcgatgcgc
     1801 agttcatcgc attactgatc gttgccaacc agtacggcct taatccgtgg acgaaagaaa
     1861 tttacgcctt tcctgataag cagaatggca tcgttccggt ggtgggcgtt gatggctggt
     1921 cccgcatcat caatgaaaac cagcagtttg atggcatgga ctttgagcag gacaatgaat
     1981 cctgtacatg ccggatttac cgcaaggacc gtaatcatcc gatctgcgtt accgaatgga
     2041 tggatgaatg ccgccgcgaa ccattcaaaa ctcgcgaagg cagagaaatc acggggccgt
     2101 ggcagtcgca tcccaaacgg atgttacgtc ataaagccat gattcagtgt gcccgtctgg
     2161 ccttcggatt tgctggtatc tatgacaagg atgaagccga gcgcattgtc gaaaatactg
     2221 catacactgc agaacgtcag ccggaacgcg acatcactcc ggttaacgat gaaaccatgc
     2281 aggagattaa cactctgctg atcgccctgg ataaaacatg ggatgacgac ttattgccgc
     2341 tctgttccca gatatttcgc cgcgacattc gtgcatcgtc agaactgaca caggccgaag
     2401 cagtaaaagc tcttggattc ctgaaacaga aagccgcaga gcagaaggtg gcagcatgac
     2461 accggacatt atcctgcagc gtaccgggat cgatgtgaga gctgtcgaac agggggatga
     2521 tgcgtggcac aaattacggc tcggcgtcat caccgcttca gaagttcaca acgtgatagc
     2581 aaaaccccgc tccggaaaga agtggcctga catgaaaatg tcctacttcc acaccctgct
     2641 tgctgaggtt tgcaccggtg tggctccgga agttaacgct aaagcactgg cctggggaaa
     2701 acagtacgag aacgacgcca gaaccctgtt tgaattcact tccggcgtga atgttactga
     2761 atccccgatc atctatcgcg acgaaagtat gcgtaccgcc tgctctcccg atggtttatg
     2821 cagtgacggc aacggccttg aactgaaatg cccgtttacc tcccgggatt tcatgaagtt
     2881 ccggctcggt ggtttcgagg ccataaagtc agcttacatg gcccaggtgc agtacagcat
     2941 gtgggtgacg cgaaaaaatg cctggtactt tgccaactat gacccgcgta tgaagcgtga
     3001 aggcctgcat tatgtcgtga ttgagcggga tgaaaagtac atggcgagtt ttgacgagat
     3061 cgtgccggag ttcatcgaaa aaatggacga ggcactggct gaaattggtt ttgtatttgg
     3121 ggagcaatgg cgatgacgca tcctcacgat aatatccggg taggcgcaat cactttcgtc
     3181 tactccgtta caaagcgagg ctgggtattt cccggccttt ctgttatccg aaatccactg
     3241 aaagcacagc ggctggctga ggagataaat aataaacgag gggctgtatg cacaaagcat
     3301 cttctgttga gttaagaacg agtatcgaga tggcacatag ccttgctcaa attggaatca
     3361 ggtttgtgcc aataccagta gaaacagacg aagaatccat ggggcatggc gaattaatct
     3421 ttctgcgaat tgagatgacg ccactggctg ggcgtcatcc cggtttcccg ggtaaacacc
     3481 accgaaaaat agttactatc ttcaaagcca cattcggtcg aaatatcact gattaacagg
     3541 cggctatgct ggagaagata ttgcgcatga cacactctga cctgtcgcag atattgattg
     3601 atggtcattc cagtctgctg gcgaaattgc tgacgcaaaa cgcgctcact gcacgatgcc
     3661 tcatcacaaa atttatccag cgcaaaggga cttttcaggc tagccgccag ccgggtaatc
     3721 agcttatcca gcaacgtttc gctggatgtt ggcggcaacg aatcactggt gtaacgatgg
     3781 cgattcagca acatcaccaa ctgcccgaac agcaactcag ccatttcgtt agcaaacggc
     3841 acatgctgac tactttcatg ctcaagctga ccgataacct gccgcgcctg cgccatcccc
     3901 atgctaccta agcgccagtg tggttgccct gcgctggcgt taaatcccgg aatcgccccc
     3961 tgccagtcaa gattcagctt cagacgctcc gggcaataaa taatattctg caaaaccaga
     4021 tcgttaacgg aagcgtagga gtgtttatcg tcagcatgaa tgtaaaagag atcgccacgg
     4081 gtaatgcgat aagggcgatc gttgagtaca tgcaggccat taccgcgcca gacaatcacc
     4141 agctcacaaa aatcatgtgt atgttcagca aagacatctt gcggataacg gtcagccaca
     4201 gcgactgcct gctggtcgct ggcaaaaaaa tcatctttga gaagttttaa ctgatgcgcc
     4261 accgtggcta cctcggccag agaacgaagt tgattattcg caatatggcg tacaaatacg
     4321 ttgagaagat tcgcgttatt gcagaaagcc atcccgtccc tggcgaatat cacgcggtga
     4381 ccagttaaac tctcggcgaa aaagcgtcga aaagtggtta ctgtcgctga atccacagcg
     4441 ataggcgatg tcagtaacgc tggcctcgct gtggcgtagc agatgtcggg ctttcatcag
     4501 tcgcaggcgg ttcaggtatc gctgaggcgt cagtcccgtt tgctgcttaa gctgccgatg
     4561 tagcgtacgc agtgaaagag aaaattgatc cgccacggca tcccaattca cctcatcggc
     4621 aaaatggtcc tccagccagg ccagaagcaa gttgagacgt gatgcgctgt tttccaggtt
     4681 ctcctgcaaa ctgcttttac gcagcaagag cagtaattgc ataaacaaga tctcgcgact
     4741 ggcggtcgag ggtaaatcat tttccccttc ctgctgttcc atctgtgcaa ccagctgtcg
     4801 cacctgctgc aatacgctgt ggttaacgcg ccagtgagac ggatactgcc catccagctc
     4861 ttgtggcagc aactgattca gcccggcgag aaactgaaat cgatccggcg agcgatacag
     4921 cacattggtc agacacagat tatcggtatg ttcatacaga tgccgatcat gatcgcgtac
     4981 gaaacagacc gtgccaccgg tgatggtata gggctgccca ttaaacacat gaatacccgt
     5041 gccatgttcg acaatcacaa tttcatgaaa atcatgatga tgttcaggaa aatccgcctg
     5101 cgggagccgg ggttctatcg ccacggacgc gttaccagac ggaaaaaaat ccacactatg
     5161 taatacggtc atactggcct cctgatgtcg tcaacacggc gaaatagtaa tcacgaggtc
     5221 aggttcttac cttaaatttt cgacggaaaa ccacgtaaaa aacgtcgatt tttcaagata
     5281 cagcgtgaat tttcaggaaa tgcggtgagc atcacatcac cacaattcag caaattgtga
     5341 acatcatcac gttcatcttt ccctggttgc caatggccca ttttcctgtc agtaacgaga
     5401 aggtcgcgaa ttcaggcgct ttttagactg gtcgtaatga aattcagcag gatcacatat
     5461 gcatcaaaaa aaccaggtaa tgaacctggg tccgaactct aaactgctga aagaatacaa
     5521 atcccagctg atcgaactga acatcgaaca gttcgaagca ggtatcggtc tgatcctggg
     5581 tgatgcttac atccgttctc gtgatgaagg taaaacctac tgtatgcagt tcgagtggaa
     5641 aaacaaagca tacatggacc acgtatgtct gctgtacgat cagtgggtac tgtctttcaa
     5701 acaccaagct ttcaacaaac tggctaacct gttcatcgtt aacaacaaaa aaaccatccc
     5761 gaacaacctg gttgaaaact acctgacccc gatgtctctg gcatactggt tcatggatga
     5821 tggtggtaaa tgggattaca acaaaaactc taccaacaaa tcgatcgtac tgaacaccca
     5881 gtctttcact ttcgaagaag tagaatacct ggttaagggt ctgcgtaaca aattccaact
     5941 gaactgttac gtaaaaatca acaaaaacaa accgatcatc tacatcgatt ctatgtctta
     6001 cctgatcttc tacaacctga tcaaaccgta cctgatcccg cagatgatgt acaaactgcc
     6061 gaacactatc tcctccgaaa ctttcctgaa ataataacca tgggtatgga cagttttccc
     6121 tttgatatgt aacggtgaac agttgttcta cttttgtttg ttagtcttga tgcttcactg
     6181 atagatacaa gagccataag aacctcagat ccttccgtat ttagccagta tgttctctag
     6241 tgtggttcgt tgtttttgcg tgagccatga gaacgaacca ttgagatcat acttactttg
     6301 catgtcactc aaaaattttg cctcaaaact ggtgagctga atttttgcag ttaaagcatc
     6361 gtgtagtgtt tttcttagtc cgttacgtag gtaggaatct gatgtaatgg ttgttggtat
     6421 tttgtcacca ttcattttta tctggttgtt ctcaagttcg gttacgagat ccatttgtct
     6481 atctagttca acttggaaaa tcaacgtatc agtcgggcgg cctcgcttat caaccaccaa
     6541 tttcatattg ctgtaagtgt ttaaatcttt acttattggt ttcaaaaccc attggttaag
     6601 ccttttaaac tcatggtagt tattttcaag cattaacatg aacttaaatt catcaaggct
     6661 aatctctata tttgccttgt gagttttctt ttgtgttagt tcttttaata accactcata
     6721 aatcctcata gagtatttgt tttcaaaaga cttaacatgt tccagattat attttatgaa
     6781 tttttttaac tggaaaagat aaggcaatat ctcttcacta aaaactaatt ctaatttttc
     6841 gcttgagaac ttggcatagt ttgtccactg gaaaatctca aagcctttaa ccaaaggatt
     6901 cctgatttcc acagttctcg tcatcagctc tctggttgct ttagctaata caccataagc
     6961 attttcccta ctgatgttca tcatctgagc gtattggtta taagtgaacg ataccgtccg
     7021 ttctttcctt gtagggtttt caatcgtggg gttgagtagt gccacacagc ataaaattag
     7081 cttggtttca tgctccgtta agtcatagcg actaatcgct agttcatttg ctttgaaaac
     7141 aactaattca gacatacatc tcaattggtc taggtgattt taatcactat accaattgag
     7201 atgggctagt caatgataat tactagtcct tttcctttga gttgtgggta tctgtaaatt
     7261 ctgctagacc tttgctggaa aacttgtaaa ttctgctaga ccctctgtaa attccgctag
     7321 acctttgtgt gttttttttg tttatattca agtggttata atttatagaa taaagaaaga
     7381 ataaaaaaag ataaaaagaa tagatcccag ccctgtgtat aactcactac tttagtcagt
     7441 tccgcagtat tacaaaagga tgtcgcaaac gctgtttgct cctctacaaa acagacctta
     7501 aaaccctaaa ggcttaagta gcaccctcgc aagctcggtt gcggccgcaa tcgggcaaat
     7561 cgctgaatat tccttttgtc tccgaccatc aggcacctga gtcgctgtct ttttcgtgac
     7621 attcagttcg ctgcgctcac ggctctggca gtgaatgggg gtaaatggca ctacaggcgc
     7681 cttttatgga ttcatgcaag gaaactaccc ataatacaag aaaagcccgt cacgggcttc
     7741 tcagggcgtt ttatggcggg tctgctatgt ggtgctatct gactttttgc tgttcagcag
     7801 ttcctgccct ctgattttcc agtctgacca cttcggatta tcccgtgaca ggtcattcag
     7861 actggctaat gcacccagta aggcagcggt atcatcaacg gggtctgacg ctcagtggaa
     7921 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
     7981 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
     8041 tgacagttac gtttaaacca tttcctgcag gggggggggg aaagccacgt tgtgtctcaa
     8101 aatctctgat gttacattgc acaagataaa aatatatcat catgaacaat aaaactgtct
     8161 gcttacataa acagtaatac aaggggtgtt atgagccata ttcaacggga aacgtcttgc
     8221 tcgaggccgc gattaaattc caacatggat gctgatttat atgggtataa atgggctcgc
     8281 gataatgtcg ggcaatcagg tgcgacaatc tatcgattgt atgggaagcc cgatgcgcca
     8341 gagttgtttc tgaaacatgg caaaggtagc gttgccaatg atgttacaga tgagatggtc
     8401 agactaaact ggctgacgga atttatgcct cttccgacca tcaagcattt tatccgtact
     8461 cctgatgatg catggttact caccactgcg atccccggga aaacagcatt ccaggtatta
     8521 gaagaatatc ctgattcagg tgaaaatatt gttgatgcgc tggcagtgtt cctgcgccgg
     8581 ttgcattcga ttcctgtttg taattgtcct tttaacagcg atcgcgtatt tcgtctcgct
     8641 caggcgcaat cacgaatgaa taacggtttg gttgatgcga gtgattttga tgacgagcgt
     8701 aatggctggc ctgttgaaca agtctggaaa gaaatgcata agcttttgcc attctcaccg
     8761 gattcagtcg tcactcatgg tgatttctca cttgataacc ttatttttga cgaggggaaa
     8821 ttaataggtt gtattgatgt tggacgagtc ggaatcgcag accgatacca ggatcttgcc
     8881 atcctatgga actgcctcgg tgagttttct ccttcattac agaaacggct ttttcaaaaa
     8941 tatggtattg ataatcctga tatgaataaa ttgcagtttc atttgatgct cgatgagttt
     9001 ttctaatcag aattggttaa ttggttgg
//
