LOCUS       sequence-151950-         824 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway destination vector for S. pombe. Allow N-terminal tag(s) to 
            be introduced using MCS. attR1-CmR-ccdB-attR2 cassette, nmt1 
            (ATATATAAA) promoter, ura4+ selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 824)
  TITLE     Akusjarvi and Bjerling plasmids
REFERENCE   2  (bases 1 to 824)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pREP4xN_ccdB
FEATURES             Location/Qualifiers
     source          1..824
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(join(86..92,93..110,111..116))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(141..265)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 acggtggtat atccagtgat ttttttctcc attttagctt ccttagctcc tgaaaatctc
       61 gacggatcct aactcaaaat ccacacatta tacgagccgg aagcataaag tgtaaagcct
      121 ggggtgccta atgcggccgc catagtgact ggatatgttg tgttttacag tattatgtag
      181 tctgtttttt atgcaaaatc taatttaata tattgatatt tatatcattt tacgtttctc
      241 gttcagcttt tttgtacaaa cttgtcatat gactagtctc gaggccaatt taacaaagcg
      301 actataagtc agaaagtgag aatgagattg aaataattaa ttcaacttat tcaattgatg
      361 atatgccagg attcctcttc ctttatatat cttcaatgtt tcgattaaca ggtccattat
      421 ccggttttta gtttcgctgc acattgccga atgacaatcg gattccttta agctttgctt
      481 ttgccctgtt ttctctcccc agtaatcgtt gggtgactga accatccagt agccgagagt
      541 tacgtttttc ctgatttaat aaatatccat atctatttgt ctcgtggtgt tttacagcaa
      601 ctaaccgaaa ttactatttt gcatcatcca accataaaga gacaacaata attctagaaa
      661 acatataaca agtgtttttt tctatttacg tctcatacgc aaagtccact ttaccatgtt
      721 ttggaaactt gtacccaacc attcattttt cgattttgta atgaggctct tttcttctaa
      781 caatcattga ggtcattatc atgtgtcctc acctattgtt taaa
//
