LOCUS       sequence-152000-        1096 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  DULIP Bait Destination Vector (Gateway, C-terminal).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1096)
  AUTHORS   Trepte P, Buntru A, Klockmeier K, Willmore L, Arumughan A, Secker C,
            Zenkner M, Brusendorf L, Rau K, Redel A, Wanker EE
  TITLE     DULIP: A Dual Luminescence-Based Co-Immunoprecipitation Assay for 
            Interactome Mapping in Mammalian Cells.
  JOURNAL   J Mol Biol. 2015 Oct 23;427(21):3375-88. doi: 
            10.1016/j.jmb.2015.08.003. Epub 2015 Aug 8.
  PUBMED    26264872
REFERENCE   2  (bases 1 to 1096)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.jmb.2015.08.003"; journalName: "J Mol Biol"; date: 
            "2015-10-23- 23"; volume: "427"; issue: "21"; pages: "3375-88"
COMMENT     Sequence Label: pGW-RL-PA
FEATURES             Location/Qualifiers
     source          1..1096
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(35..208)
                     /codon_start=1
                     /product="IgG-binding unit of Staphylococcus aureus protein
                     A"
                     /label=ProtA
                     /translation="VDNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLKDDPSQSANLL
                     AEAKKLNGAQAPK"
     CDS             complement(209..382)
                     /codon_start=1
                     /product="IgG-binding unit of Staphylococcus aureus protein
                     A"
                     /label=ProtA
                     /translation="VDNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLKDDPSQSANLL
                     AEAKKLNDAQAPK"
ORIGIN
        1 gccctctaga ctcgagtcac gagttcgcgt ctactttcgg cgcctgagca ccatttagct
       61 ttttagcttc tgctaaaagg ttagcgcttt ggcttgggtc atcttttaaa ctttggatga
      121 aggcgtttcg ttgttcttcg tttaagttag gtaaatgtaa gatctcatag aacgcgtttt
      181 gttgttcttt gttgaatttg ttgtctactt tcggcgcctg agcatcattt agctttttag
      241 cttctgctaa aaggttagcg ctttggcttg ggtcatcttt taaactttgg atgaaggcgt
      301 ttcgttgttc ttcgtttaag ttaggtaaat gtaagatctc atagaacgcg ttttgttgtt
      361 ctttgttgaa tttgttgtcc accatcgagc ccgagccgaa ttcctgctcg ttcttcagca
      421 cgcgctccac gaagctcttg atgtacttac ccatttcatc tggagcgtcc tcctggctga
      481 agtggaggcc cttcaccttc acgaactcgg tgttagggaa cttcttagct ccctcgacaa
      541 tagcgttgga aaagaaccca gggtcggact cgatgaacat cttaggcaga tcgtcgctgg
      601 cccgaaggta ggcgttgtag ttgcggacaa tctggacgac gtcgggcttg cctcccttaa
      661 cgagagggat ctcgcgaggc caggagaggg taggccgtct aacctcgccc ttctccttga
      721 atggctccag gtaggcagcg aactcctcag gctccagttt ccgcatgatc ttgcttggga
      781 gcatggtctc gacgaagaag ttattctcaa gcaccatttt ctcgccctct tcgctcttga
      841 tcagggcgat atcctcctcg atgtcaggcc actcgtccca ggactcgatc acgtccacga
      901 cactctcagc atggacgatg gccttgatct tgtcttggtg ctcgtaggag tagtgaaagg
      961 ccagacaagc cccccagtcg tggcccacaa agatgatttt ctttggaagg ttcagcagct
     1021 cgaaccaagc ggtgaggtac ttgtagtgat ccaggaggcg atatgagcca ttcccgctct
     1081 tgccggactt acccat
//
