LOCUS       sequence-152068-       11196 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  3rd generation lentiviral vector; TetON promoter driving human Ngn2;
            constitutive expression of Puromycin selection marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11196)
  AUTHORS   Busskamp V, Lewis NE, Guye P, Ng AH, Shipman SL, Byrne SM, Sanjana 
            NE, Murn J, Li Y, Li S, Stadler M, Weiss R, Church GM
  TITLE     Rapid neurogenesis through transcriptional activation in human stem 
            cells.
  JOURNAL   Mol Syst Biol. 2014 Nov 17;10(11):760. doi: 10.15252/msb.20145508.
  PUBMED    25403753
REFERENCE   2  (bases 1 to 11196)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Syst 
            Biol."; date: "2014-11-17"; volume: "10(11):760. doi"; pages: " 
            10.15252/msb.20145508"
COMMENT     Sequence Label: pLV_TRET_hNgn2_UBC_Puro
FEATURES             Location/Qualifiers
     source          1..11196
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             212..392
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(418..435)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    424..648
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      694..1122
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(781..800)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     991..1012
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(1131..1151)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        1136..1465
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      1316..1451
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     1378..1397
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        1513..1560
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             1579..1953
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    2083..2204
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2120..2139)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2174..2193
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(2253..2269)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2253..2269)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2266..2288)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2277..2293
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2301..2307,2308..2325,2326..2331))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2346..2367
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2484..2501)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2655..3243)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2735..2754)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3414..4205,4206..4274))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4037..4056
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4275..4379)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(4454..4473)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        4645..5024
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5026..5224
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             5242..5422
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    5469..5594
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    6087..6320
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             6505..6549
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    6847..6964
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    7040..7060
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    7105..7123
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7140..7158
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7176..7194
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7212..7230
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7247..7265
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7283..7301
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        7313..7351
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     7348..7372
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    7391..7415
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(8246..8270)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             8323..8364
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     promoter        8379..9590
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     9427..9446
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             9643..10242
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(9643..9662)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     10139..10159
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    10295..10883
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(10348..10368)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(10766..10777)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(10886..10902)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10887..10903)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
ORIGIN
        1 cagatatcca ctgacctttg gatggtgcta caagctagta ccagttgagc aagagaaggt
       61 agaagaagcc aatgaaggag agaacacccg cttgttacac cctgtgagcc tgcatgggat
      121 ggatgacccg gagagagaag tattagagtg gaggtttgac agccgcctag catttcatca
      181 catggcccga gagctgcatc cggactgtac tgggtctctc tggttagacc agatctgagc
      241 ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg
      301 agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag
      361 acccttttag tcagtgtgga aaatctctag cagggcccgt ttaaacccgc tgatcagcct
      421 cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga
      481 ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt
      541 gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg
      601 attgggaaga caatagcagg catgctgggg atgcggtggg ctctatggct tctgaggcgg
      661 aaagaaccag ctggggctct agggggtatc cccacgcgcc ctgtagcggc gcattaagcg
      721 cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg
      781 ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc
      841 taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa
      901 aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc
      961 ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac
     1021 tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt
     1081 ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttaattctgt ggaatgtgtg
     1141 tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
     1201 tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
     1261 gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc
     1321 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat
     1381 ttatgcagag gccgaggccg cctctgcctc tgagctattc cagaagtagt gaggaggctt
     1441 ttttggaggc ctaggctttt gcaaaaagct cccgggagct tgtatatcca ttttcggatc
     1501 tgatcagcac gtgttgacaa ttaatcatcg gcatagtata tcggcatagt ataatacgac
     1561 aaggtgagga actaaaccat ggccaagttg accagtgccg ttccggtgct caccgcgcgc
     1621 gacgtcgccg gagcggtcga gttctggacc gaccggctcg ggttctcccg ggacttcgtg
     1681 gaggacgact tcgccggtgt ggtccgggac gacgtgaccc tgttcatcag cgcggtccag
     1741 gaccaggtgg tgccggacaa caccctggcc tgggtgtggg tgcgcggcct ggacgagctg
     1801 tacgccgagt ggtcggaggt cgtgtccacg aacttccggg acgcctccgg gccggccatg
     1861 accgagatcg gcgagcagcc gtgggggcgg gagttcgccc tgcgcgaccc ggccggcaac
     1921 tgcgtgcact tcgtggccga ggagcaggac tgacacgtgc tacgagattt cgattccacc
     1981 gccgccttct atgaaaggtt gggcttcgga atcgttttcc gggacgccgg ctggatgatc
     2041 ctccagcgcg gggatctcat gctggagttc ttcgcccacc ccaacttgtt tattgcagct
     2101 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
     2161 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctgtataccg
     2221 tcgacctcta gctagagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt
     2281 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt
     2341 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg
     2401 ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg
     2461 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
     2521 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
     2581 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     2641 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     2701 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     2761 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     2821 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
     2881 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     2941 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     3001 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     3061 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg
     3121 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     3181 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     3241 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
     3301 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
     3361 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
     3421 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
     3481 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
     3541 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
     3601 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
     3661 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
     3721 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
     3781 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
     3841 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
     3901 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
     3961 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
     4021 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
     4081 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
     4141 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
     4201 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
     4261 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
     4321 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
     4381 acatttcccc gaaaagtgcc acctgacgtc gacggatcgg gagatctccc gatcccctat
     4441 ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat ctgctccctg
     4501 cttgtgtgtt ggaggtcgct gagtagtgcg cgagcaaaat ttaagctaca acaaggcaag
     4561 gcttgaccga caattgcatg aagaatctgc ttagggttag gcgttttgcg ctgcttcgcg
     4621 atgtacgggc cagatatacg cgttgacatt gattattgac tagttattaa tagtaatcaa
     4681 ttacggggtc attagttcat agcccatata tggagttccg cgttacataa cttacggtaa
     4741 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     4801 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     4861 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     4921 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
     4981 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
     5041 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
     5101 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
     5161 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
     5221 gcagcgcgtt ttgcctgtac tgggtctctc tggttagacc agatctgagc ctgggagctc
     5281 tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa
     5341 gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag
     5401 tcagtgtgga aaatctctag cagtggcgcc cgaacaggga cttgaaagcg aaagggaaac
     5461 cagaggagct ctctcgacgc aggactcggc ttgctgaagc gcgcacggca agaggcgagg
     5521 ggcggcgact ggtgagtacg ccaaaaattt tgactagcgg aggctagaag gagagagatg
     5581 ggtgcgagag cgtcagtatt aagcggggga gaattagatc gcgatgggaa aaaattcggt
     5641 taaggccagg gggaaagaaa aaatataaat taaaacatat agtatgggca agcagggagc
     5701 tagaacgatt cgcagttaat cctggcctgt tagaaacatc agaaggctgt agacaaatac
     5761 tgggacagct acaaccatcc cttcagacag gatcagaaga acttagatca ttatataata
     5821 cagtagcaac cctctattgt gtgcatcaaa ggatagagat aaaagacacc aaggaagctt
     5881 tagacaagat agaggaagag caaaacaaaa gtaagaccac cgcacagcaa gcggccgctg
     5941 atcttcagac ctggaggagg agatatgagg gacaattgga gaagtgaatt atataaatat
     6001 aaagtagtaa aaattgaacc attaggagta gcacccacca aggcaaagag aagagtggtg
     6061 cagagagaaa aaagagcagt gggaatagga gctttgttcc ttgggttctt gggagcagca
     6121 ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac aggccagaca attattgtct
     6181 ggtatagtgc agcagcagaa caatttgctg agggctattg aggcgcaaca gcatctgttg
     6241 caactcacag tctggggcat caagcagctc caggcaagaa tcctggctgt ggaaagatac
     6301 ctaaaggatc aacagctcct ggggatttgg ggttgctctg gaaaactcat ttgcaccact
     6361 gctgtgcctt ggaatgctag ttggagtaat aaatctctgg aacagatttg gaatcacacg
     6421 acctggatgg agtgggacag agaaattaac aattacacaa gcttaataca ctccttaatt
     6481 gaagaatcgc aaaaccagca agaaaagaat gaacaagaat tattggaatt agataaatgg
     6541 gcaagtttgt ggaattggtt taacataaca aattggctgt ggtatataaa attattcata
     6601 atgatagtag gaggcttggt aggtttaaga atagtttttg ctgtactttc tatagtgaat
     6661 agagttaggc agggatattc accattatcg tttcagaccc acctcccaac cccgagggga
     6721 cccgacaggc ccgaaggaat agaagaagaa ggtggagaga gagacagaga cagatccatt
     6781 cgattagtga acggatcggc actgcgtgcg ccaattctgc agacaaatgg cagtattcat
     6841 ccacaatttt aaaagaaaag gggggattgg ggggtacagt gcaggggaaa gaatagtaga
     6901 cataatagca acagacatac aaactaaaga attacaaaaa caaattacaa aaattcaaaa
     6961 ttttcgggtt tattacaggg acagcagaga tccagtttgg ttaattaagt atcgatgtcg
     7021 acgttaacgc tagtgatatc aactttgtat agaaaagttg gctccgaatt cgcccttcag
     7081 gtccgaggtt ctagacgagt ttactcccta tcagtgatag agaacgatgt cgagtttact
     7141 ccctatcagt gatagagaac gtatgtcgag tttactccct atcagtgata gagaacgtat
     7201 gtcgagttta ctccctatca gtgatagaga acgtatgtcg agtttatccc tatcagtgat
     7261 agagaacgta tgtcgagttt actccctatc agtgatagag aacgtatgtc gaggtaggcg
     7321 tgtacggtgg gaggcctata taagcagagc tcgtttagtg aaccgtcaga tcgcaaaggg
     7381 cgaattcgac ccaagtttgt acaaaaaagc aggctgacct ccaccatgtt cgtcaaatcc
     7441 gagaccttgg agttgaagga ggaagaggac gtgttagtgc tgctcggatc ggcctccccc
     7501 gccttggcgg ccctgacccc gctgtcatcc agcgccgacg aagaagagga ggaggagccg
     7561 ggcgcgtcag gcggggcgcg tcggcagcgc ggggctgagg ccgggcaggg ggcgcggggc
     7621 ggcgtggctg cgggtgcgga gggctgccgg cccgcacggc tgctgggtct ggtacacgat
     7681 tgcaaacggc gcccttcccg ggcgcgggcc gtctcccgag gcgccaagac ggccgagacg
     7741 gtgcagcgca tcaagaagac ccgtagactg aaggccaaca accgcgagcg aaaccgcatg
     7801 cacaacctca acgcggcact ggacgcgctg cgcgaggtgc tccccacgtt ccccgaggac
     7861 gccaagctca ccaagatcga gaccctgcgc ttcgcccaca actacatctg ggcactcacc
     7921 gagaccctgc gcctggcgga tcactgcggg ggcggcggcg ggggcctgcc gggggcgctc
     7981 ttctccgagg cagtgttgct gagcccggga ggcgccagcg ccgccctgag cagcagcgga
     8041 gacagcccct cgcccgcctc cacgtggagt tgcaccaaca gccccgcgcc gtcctcctcc
     8101 gtgtcctcca attccacctc cccctacagc tgcactttat cgcccgccag cccggccggg
     8161 tcagacatgg actattggca gcccccacct cccgacaagc accgctatgc acctcacctc
     8221 cccatagcca gggattgtat ctagtaccca gctttcttgt acaaagtggt tgatatccag
     8281 cacagtggcg gccgctcgag tctagagggc ccgcggttcg aaggtaagcc tatccctaac
     8341 cctctcctcg gtctcgattc tacgcgtacc ggcgatctgg cctccgcgcc gggttttggc
     8401 gcctcccgcg ggcgcccccc tcctcacggc gagcgctgcc acgtcagacg aagggcgcag
     8461 cgagcgtcct gatccttccg cccggacgct caggacagcg gcccgctgct cataagactc
     8521 ggccttagaa ccccagtatc agcagaagga cattttagga cgggacttgg gtgactctag
     8581 ggcactggtt ttctttccag agagcggaac aggcgaggaa aagtagtccc ttctcggcga
     8641 ttctgcggag ggatctccgt ggggcggtga acgccgatga ttatataagg acgcgccggg
     8701 tgtggcacag ctagttccgt cgcagccggg atttgggtcg cggttcttgt ttgtggatcg
     8761 ctgtgatcgt cacttggtga gtagcgggct gctgggctgg ccggggcttt cgtggccgcc
     8821 gggccgctcg gtgggacgga agcgtgtgga gagaccgcca agggctgtag tctgggtccg
     8881 cgagcaaggt tgccctgaac tgggggttgg ggggagcgca gcaaaatggc ggctgttccc
     8941 gagtcttgaa tggaagacgc ttgtgaggcg ggctgtgagg tcgttgaaac aaggtggggg
     9001 gcatggtggg cggcaagaac ccaaggtctt gaggccttcg ctaatgcggg aaagctctta
     9061 ttcgggtgag atgggctggg gcaccatctg gggaccctga cgtgaagttt gtcactgact
     9121 ggagaactcg gtttgtcgtc tgttgcgggg gcggcagtta tggcggtgcc gttgggcagt
     9181 gcacccgtac ctttgggagc gcgcgccctc gtcgtgtcgt gacgtcaccc gttctgttgg
     9241 cttataatgc agggtggggc cacctgccgg taggtgtgcg gtaggctttt ctccgtcgca
     9301 ggacgcaggg ttcgggccta gggtaggctc tcctgaatcg acaggcgccg gacctctggt
     9361 gaggggaggg ataagtgagg cgtcagtttc tttggtcggt tttatgtacc tatcttctta
     9421 agtagctgaa gctccggttt tgaactatgc gctcggggtt ggcgagtgtg ttttgtgaag
     9481 ttttttaggc accttttgaa atgtaatcat ttgggtcaat atgtaatttt cagtgttaga
     9541 ctagtaaatt gtccgctaaa ttctggccgt ttttggcttt tttgttagac gaagcttggg
     9601 ctgcaggtcg actctagagg atccccgggt agaccagcca ccatgaccga gtacaagccc
     9661 acggtgcgcc tcgccacccg cgacgacgtc ccccgggccg tacgcaccct cgccgccgcg
     9721 ttcgccgact accccgccac gcgccacacc gtcgacccgg accgccacat cgagcgggtc
     9781 accgagctgc aagaactctt cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc
     9841 gcggacgacg gcgccgcggt ggcggtctgg accacgccgg agagcgtcga agcgggggcg
     9901 gtgttcgccg agatcggccc gcgcatggcc gagttgagcg gttcccggct ggccgcgcag
     9961 caacagatgg aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc
    10021 accgtcggcg tctcgcccga ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc
    10081 ggagtggagg cggccgagcg cgccggggtg cccgccttcc tggagacctc cgcgccccgc
    10141 aacctcccct tctacgagcg gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa
    10201 ggaccgcgca cctggtgcat gacccgcaag cccggtgcct gaggctaagc acaattcgag
    10261 ctcggtaccg caattcgata tcaagcttat cgataatcaa cctctggatt acaaaatttg
    10321 tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc
    10381 tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta
    10441 taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt
    10501 ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca
    10561 gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc
    10621 ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt
    10681 gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt gttgccacct ggattctgcg
    10741 cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg
    10801 cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat
    10861 ctccctttgg gccgcctccc cgcatcgata ccgtcgacct cgagacctag aaaaacatgg
    10921 agcaatcaca agtagcaata cagcagctac caatgctgat tgtgcctggc tagaagcaca
    10981 agaggaggag gaggtgggtt ttccagtcac acctcaggta cctttaagac caatgactta
    11041 caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat
    11101 tcactcccaa cgaagacaag atatccttga tctgtggatc taccacacac aaggctactt
    11161 ccctgattgg cagaactaca caccagggcc agggat
//
