LOCUS       sequence-152107-         885 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Creating LexA::GADfluw lines from MiMIC lines with inserts into 
            coding introns in Phase 2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 885)
  AUTHORS   Diao F, Ironfield H, Luan H, Diao F, Shropshire WC, Ewer J, Marr E, 
            Potter CJ, Landgraf M, White BH
  TITLE     Plug-and-play genetic access to drosophila cell types using 
            exchangeable exon cassettes.
  JOURNAL   Cell Rep. 2015 Mar 3;10(8):1410-21. doi: 
            10.1016/j.celrep.2015.01.059. Epub 2015 Feb 26.
  PUBMED    25732830
REFERENCE   2  (bases 1 to 885)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2015.01.059"; journalName: "Cell Rep"; date: 
            "2015-03-3- 3"; volume: "10"; issue: "8"; pages: "1410-21"
COMMENT     Sequence Label: pBS-KS-attB2-SA(2)-T2A-LexA::GADfluw-Hsp70
FEATURES             Location/Qualifiers
     source          1..885
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    47..116
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     CDS             284..337
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             344..364
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 ctcactatag ggcgaattgg agctcgctag cgatgtaggt cacggtctcg aagccgcggt
       61 gcgggtgcca gggcgtgccc ttgggctccc cgggcgcgta ctccacctca cccatctggt
      121 ccatcatgat ggtcgactct agaagtcgat ccaacatggc gacttgtccc atccccggca
      181 tgtttaaata tactaattat tcttgaacta attttaatca accgatttat ctctcttccg
      241 caggtcggga ggttccggtg gaagcggagg tagcggcgga tccgagggcc gcggcagcct
      301 gctgacctgc ggcgatgtgg aggagaaccc cgggcccatg ccacccaaga agaagcgaaa
      361 agtagaagat ccaatgaagg ctctcacggc ccgacaacag gaagtttttg atttgatacg
      421 ggatcatata tcccaaacgg gtatgcctcc gacccgcgca gagatagcac agcgactggg
      481 ctttcgatcg cctaacgccg cggaggagca cttgaaggca ctggcccgca agggtgtcat
      541 tgaaatcgtg tccggtgcga gccgcggaat ccggctgttg caggaggagg aagagggcct
      601 gccactggtg ggacgcgtgg ccgctggcga gccgctgctg gcccagcaac acatagaggg
      661 acactatcag gtggacccct ccttgtttaa gccaaatgct gatttcctgt tgcgggtgtc
      721 gggaatgtcc atgaaggaca tcggtattat ggatggtgac ctcctcgccg tccataagac
      781 acaggatgtc aggaacggcc aggtagtcgt tgccaggata gacgatgagg tcactgtgaa
      841 acgtctcaag aagcaaggca ataaggtcga gctgctgccg gagaa
//
