LOCUS       sequence-152177-         868 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Doxycycline-inducible lentiviral overexpression of human MYOD1 ORF 
            with a FLAG tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 868)
  TITLE     Alexander lab Muscle Factors
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 868)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pINDUCER20-FLAG-hMYOD1 (#800)
FEATURES             Location/Qualifiers
     source          1..868
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(183..209)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    262..286
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 aggataggac gctcgctgcg cccttcgtct gacgtggcag cgctcgccgt gaggaggggg
       61 gcgcccgcgg gaggcgccaa aacccggcgc ggaggccaga tcttgggtgg gttactccag
      121 actgccttgg gaaaagcgcc tcccctaccc ggtagaattt ctagtttaat taatcattac
      181 taagcgtagt ctgggacgtc gtatgggtat tcgaaccgcg ggccctctag actcgagcgg
      241 ccgccactgt gctggatatc aaccactttg tacaagaaag ctgggtcggc gcgcccaccc
      301 tttcagagca cctggtatat cgggttgggg ttcgcacccg cagggcactg cggggcggcg
      361 tccggtgact gggtggggtc gccgctgctc tctccctcgc tgggggcggc agcctcttgc
      421 ctgcgcggag gcgactcaga aggcacgtcc gccagcagga gggcgggcgc cgcagggctc
      481 tcggtggaga tgcgctccac gatgctggac aggcagtcta ggctcgacac cgccgcactc
      541 ttcccgggcc tgggttcgct gggcgcctcg ttgtagtagg cgccttcgta gcagttccgc
      601 cgccgggcgc cgctcggggg gccgctgtag tccatcatgc cgtcggagca gttggagcgc
      661 gggctggacg cgtcggagtc gccgctgtag tgctcgccgc cgcggcccgg gggcagcggg
      721 cccggcgcat agaaggcggc tgcggcgcca gggggcgcgg cgtcctggtc gcgcagcaga
      781 gcctgcaggc cctcgatata gcggatggcg ttgcgcagga tctccacctt gggcaaccgc
      841 tggtttggat tgctcgacgt gcagcgct
//
