LOCUS       sequence-152204-         975 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Doxycycline-inducible lentiviral overexpression of human MYF5 ORF 
            with a 3xHA tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 975)
  TITLE     Alexander lab Muscle Factors
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 975)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pINDUCER20-hMYF5-3xHA (#732)
FEATURES             Location/Qualifiers
     source          1..975
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    45..69
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      90..99
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 cggccggcct actagtccag tgtggtggaa ttctgcagat atcaacaagt ttgtacaaaa
       61 aagcaggctc cgcggccgcc cccttcaccg ccaccatgga catgacggac ggctgccagt
      121 tctcaccttc tgagtacttc tacgacggct cctgcatacc gtcccccgag ggtgaatttg
      181 gggacgagtt tgtgccgcga gtggctgcct tcggagcgca caaagcagag ctgcagggct
      241 cagatgagga cgagcacgtg cgagcgccta ccggccacca ccaggctggt cactgcctca
      301 tgtgggcctg caaagcctgc aagaggaagt ccaccaccat ggatcggcgg aaggcagcca
      361 ctatgcgcga gcggaggcgc ctgaagaagg tcaaccaggc tttcgaaacc ctcaagaggt
      421 gtaccacgac caaccccaac cagaggctgc ccaaggtgga gatcctcagg aatgccatcc
      481 gctacatcga gagcctgcag gagttgctga gagagcaggt ggagaactac tatagcctgc
      541 cgggacagag ctgctcggag cccaccagcc ccacctccaa ctgctctgat ggcatgcccg
      601 aatgtaacag tcctgtctgg tccagaaaga gcagtacttt tgacagcatc tactgtcctg
      661 atgtatcaaa tgtatatgcc acagataaaa actccttatc cagcttggat tgcttatcca
      721 acatagtgga ccggatcacc tcctcagagc aacctgggtt gcctctccag gatctggctt
      781 ctctctctcc agttgccagc accgattcac agcctgcaac tccaggggct tctagttcca
      841 ggcttatcta tcatgtgcta cgaattcgca tgcgtcgact cgagtaccca tatgacgttc
      901 cagactacgg cwttcgttaa cgagttcgaa tagcttacca ttcagagata ctggcatacg
      961 cgtaaataaa ggttg
//
