LOCUS       sequence-152245-         939 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Experimental plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 939)
  AUTHORS   Pulido-Quetglas C, Aparicio-Prat E, Arnan C, Polidori T, Hermoso T, 
            Palumbo E, Ponomarenko J, Guigo R, Johnson R
  TITLE     Scalable Design of Paired CRISPR Guide RNAs for Genomic Deletion.
  JOURNAL   PLoS Comput Biol. 2017 Mar 2;13(3):e1005341. doi: 
            10.1371/journal.pcbi.1005341. eCollection 2017 Mar.
  PUBMED    28253259
REFERENCE   2  (bases 1 to 939)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS Comput
            Biol."; date: "2017-03-2"; volume: "13(3):e1005341. doi"; pages: " 
            10.1371/journal.pcbi.1005341. eCollection 2017 Mar"
COMMENT     Sequence Label: pDECKO-mCherry TFRC_B
FEATURES             Location/Qualifiers
     source          1..939
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    155..272
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_RNA        complement(328..403)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        complement(432..646)
                     /label=H1 promoter
                     /note="human H1 RNA promoter"
     primer_bind     complement(492..511)
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     misc_RNA        complement(653..728)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     primer_bind     complement(808..827)
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
ORIGIN
        1 gacgggcacc ggagccaatt cccactcctt tcaagaccta gaaggtccat tagctgcaaa
       61 gattcctctc tgtttaaaac tttatccatc tttgcacccg ggccccctcg agccggcgcc
      121 aaagtggatc tctgctgtcc ctgtaataaa cccgaaaatt ttgaattttt gtaatttgtt
      181 tttgtaattc tttagtttgt atgtctgttg ctattatgtc tactattctt tcccctgcac
      241 tgtacccccc aatcccccct tttcttttaa aattgtggat gaatactgcc atttgtctca
      301 agatctagtt acgccaagct taaaaaagca ccgactcggt gccacttttt caagttgata
      361 acggactagc cttattttaa cttgctattt ctagctctaa aacagccgcc acccgatatc
      421 ccggggaaag agtggtctca tacagaactt ataagactcc caaatccaaa gacatttcac
      481 gtttatggtg atttcccaga acacatagcg acatgcaaat attgcagggc gccactcccc
      541 tgtccctcac agccatcttc ctgccagggc gcacgcgcgc tgggtgttcc cgcctagtga
      601 cactgggccc gcgattcctt ggagcgggtt gatgacgtca gcgttcaaaa aagcaccgac
      661 tcggtgccac tttttcaagt tgataacgga ctagccttat tttaacttgc tatttctagc
      721 tctaaaactt ttgtatttct gaggctgccg gtgtttcgtc ctttccacaa gatatataaa
      781 gccaagaaat cgaaatactt tcaagttacg gtaagcatat gatagtccat tttaaaacat
      841 aattttaaaa ctgcaaacta cccaagaaat tattactttc tacgtcacgt attttgtact
      901 aatatctttg tgtttacagt caaattaatt ctaattatc
//
