LOCUS       sequence-152301-         769 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Overexpression of human SRF ORF with a N-terminal FLAG tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 769)
  TITLE     Alexander lab Muscle Factors
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 769)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: FLAG-hSRF (#482)
FEATURES             Location/Qualifiers
     source          1..769
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        36..54
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     regulatory      89..98
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             98..121
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 accgtcagat ccgctagcga ttacgccaag ctcgaaatta accctcacta aagggaacaa
       61 aagctggagc tccaccgcgg tggcggccgc caccatggat tacaaggatg acgacgataa
      121 gagcccgggc ggatccatgt taccgaccca agctggggcc gcggcggctc tgggccgggg
      181 ctcggccctg gggggcagcc tgaaccggac cccgacgggg cggccgggcg gcggcggcgg
      241 gacacgcggg gctaacgggg gccgggtccc cgggaatggc gcggggctcg ggcccggccg
      301 cctggagcgg gaggctgcgg cagcggcggc aaccaccccg gcgcccaccg cgggggccct
      361 ctacagcggc agcgagggcg actcggagtc gggcgaggag gaggagctgg gcgccgagcg
      421 gcgcggcctg aagcggagcc tgagcgagat ggagatcggt atggtggtcg gtgggcccga
      481 ggcgtcggca gcggccaccg ggggctacgg gccggtgagc ggcgcggtga gcggggccaa
      541 gccgggtaag aagacccggg gccgcgtgaa gatcaagatg gagttcatcg acaacaagct
      601 gcggcgctac acgaccttca gcaagaggaa gacgggcatc atgaagaagg cctatgagct
      661 gtccacgctg acagggacac aggtgctgtt gctggtggcc agtgagacag gccatgtgta
      721 tacctttgcc acccgaaaac tgcagcccat gatcaccagt gagaccggc
//
