LOCUS       sequence-152342-        1039 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cas9 from S. pyogenes with 2A-Puro, and cloning backbone for sgRNA 
            (V2.0).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1039)
  AUTHORS   Ran FA, Hsu PD, Wright J, Agarwala V, Scott DA, Zhang F
  TITLE     Genome engineering using the CRISPR-Cas9 system.
  JOURNAL   Nat Protoc. 2013 Nov;8(11):2281-308. doi: 10.1038/nprot.2013.143. 
            Epub 2013 Oct 24.
  PUBMED    24157548
REFERENCE   2  (bases 1 to 1039)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nprot.2013.143"; journalName: "Nat Protoc"; date: 
            "2013-11"; volume: "8"; issue: "11"; pages: "2281-308"
COMMENT     Sequence Label: pSpCas9(BB)-2A-Puro (PX459) V2.0
FEATURES             Location/Qualifiers
     source          1..1039
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(50..97)
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
ORIGIN
        1 cgtcaccgca tgttagcaga cttcctctgc cctctccact gccgaattcc tttttctttt
       61 ttgcctggcc ggcctttttc gtggccgccg gccttttgtc gcctcccagc tgagacaggt
      121 cgatccgtgt ctcgtacagg ccggtgatgc tctggtggat cagggtggcg tccagcacct
      181 ctttggtgct ggtgtacctc ttccggtcga tggtggtgtc aaagtacttg aaggcggcag
      241 gggctcccag attggtcagg gtaaacaggt ggatgatatt ctcggcctgc tctctgatgg
      301 gcttatcccg gtgcttgttg taggcggaca gcactttgtc cagattagcg tcggccagga
      361 tcactctctt ggagaactcg ctgatctgct cgatgatctc gtccaggtag tgcttgtgct
      421 gttccacaaa cagctgtttc tgctcattat cctcggggga gcccttcagc ttctcatagt
      481 ggctggccag gtacaggaag ttcacatatt tggagggcag ggccagttcg tttcccttct
      541 gcagttcgcc ggcagaggcc agcattctct tccggccgtt ttccagctcg aacagggagt
      601 acttaggcag cttgatgatc aggtcctttt tcacttcttt gtagcccttg gcttccagaa
      661 agtcgatggg attcttctcg aagctgcttc tttccatgat ggtgatcccc agcagctctt
      721 tcacactctt cagtttcttg gacttgccct tttccacttt ggccaccacc agcacagaat
      781 aggccacggt ggggctgtcg aagccgccgt acttcttagg gtcccagtcc ttctttctgg
      841 cgatcagctt atcgctgttc ctcttgggca ggatagactc tttgctgaag ccgcctgtct
      901 gcacctcggt ctttttcacg atattcactt ggggcatgct cagcactttc cgcacggtgg
      961 caaaatcccg gcccttatcc cacacgatct ccccggtttc gccgtttgtc tcgatcagag
     1021 gccgcttccg gatctcgcc
//
