LOCUS       sequence-152466-         986 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Transcriptional unit of (human codon optimized) inactivated Cas9 
            fused to the EDLL Transcriptional Activator.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 986)
  AUTHORS   Vazquez-Vilar M, Bernabe-Orts JM, Fernandez-Del-Carmen A, Ziarsolo 
            P, Blanca J, Granell A, Orzaez D
  TITLE     A modular toolbox for gRNA-Cas9 genome engineering in plants based 
            on the GoldenBraid standard.
  JOURNAL   Plant Methods. 2016 Feb 1;12:10. doi: 10.1186/s13007-016-0101-2. 
            eCollection 2016.
  PUBMED    26839579
REFERENCE   2  (bases 1 to 986)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Methods."; date: "2016-02-1"; volume: "12:10. doi"; pages: " 
            10.1186/s13007-016-0101-2. eCollection 2016"
COMMENT     Sequence Label: pEGB 35s:dCas:EDLL:tNos (GB1190)
FEATURES             Location/Qualifiers
     source          1..986
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(15..267)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(366..386)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(390..410)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(414..434)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 gcttggcaat tcccgatcta gtaacataga tgacaccgcg cgcgataatt tatcctagtt
       61 tgcgcgctat attttgtttt ctatcgcgta ttaaatgtat aattgcggga ctctaatcat
      121 aaaaacccat ctcataaata acgtcatgca ttacatgtta attattacat gcttaacgta
      181 attcaacaga aattatatga taatcgtcgc aagaccggca acaggattca atcttaagaa
      241 actttattgc caaatgtttg aacgatcggg atcgaagatc cattccaagc tcagcgtttg
      301 cgttcttcgc tatccagcag ttcttccagc actttatcat ccagatattc aaattcaaac
      361 acttctacct tgcgcttctt ctttggatct accttcctct ttttcttcgg gtctaccttt
      421 cgcttctttt taggatccga atcagccctg ctgtctccac cgagctgaga gaggtcgatt
      481 cttgtttcat agagccccgt aattgactga tgaatcagtg tggcgtccag gacctccttt
      541 gtagaggtgt accgctttct gtctatggtg gtgtcgaagt acttgaaggc tgcaggcgcg
      601 cccaagttgg tcagagtaaa caagtggata atgttttctg cctgctccct gatgggctta
      661 tccctgtgct tattgtaagc agaaagcacc ttatcgaggt tagcgtcggc gaggatcact
      721 cttttggaga attcgcttat ttgctcgatg atctcatcaa ggtagtgttt gtgttgttcc
      781 acgaacagct gcttctgctc attatcttcg ggagatcctt tgagcttttc atagtggctg
      841 gccagataca agaaattaac gtatttagag ggcagtgcca gctcgttacc tttctgcagc
      901 tcgcccgcac tagcgagcat tcgtttccgg ccgttttcaa gctcaaagag agagtacttg
      961 ggaagcttaa tgatgaggtc tttttt
//
