LOCUS       sequence-152469-         923 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Provides the A. thaliana U6-1 RNA polIII promoter as a level 0 
            GoldenBraid part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 923)
  AUTHORS   Vazquez-Vilar M, Bernabe-Orts JM, Fernandez-Del-Carmen A, Ziarsolo 
            P, Blanca J, Granell A, Orzaez D
  TITLE     A modular toolbox for gRNA-Cas9 genome engineering in plants based 
            on the GoldenBraid standard.
  JOURNAL   Plant Methods. 2016 Feb 1;12:10. doi: 10.1186/s13007-016-0101-2. 
            eCollection 2016.
  PUBMED    26839579
REFERENCE   2  (bases 1 to 923)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Methods."; date: "2016-02-1"; volume: "12:10. doi"; pages: " 
            10.1186/s13007-016-0101-2. eCollection 2016"
COMMENT     Sequence Label: pU6-1 (GB1204)
FEATURES             Location/Qualifiers
     source          1..923
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     209..226
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(250..307)
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     misc_feature    612..633
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     terminator      636..679
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     primer_bind     complement(683..701)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctgt ggaaaaacgc
       61 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
      121 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac
      181 cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cctgcataac
      241 gcgaagtaat cttttcggtt ttaaagaaaa agggcagggt ggtgacacct tgcccttttt
      301 tgccggactg cagcggccgc tactagtcgc gatgagtggt ctcacaatca ctacttcgac
      361 tctaaccata tataaactca gctgctttct ttacctaagc gctgtggtac ttttgaagat
      421 tgttaatagc ttaaatgggc ctattttaga aaaaggccta tccctcgctt ccttctcaag
      481 cccatttata ttattgaatt ttttcccaac taaagatttt attttcttat gcaagtctca
      541 ctcacactca cgtaaatttc aaatataatc gtggtggaac taaaacaatg acctcccgag
      601 accactcatc gctctagaag cggccgcgaa ttccagaaat catccttagc gaaagctaag
      661 gatttttttt atctgaaatt ctgcctcgtg atacgcctat ttttataggt taatgtcatg
      721 ataataatgg tttcttagac gtcaggtggc actcgagttg atcgggcacg taagaggttc
      781 caactttcac cataatgaaa taagatcact accgggcgta ttttttgagt tatcgagatt
      841 ttcaggagct aaggaagcta aaatggagaa aaaaatcact ggatatacca ccgttgatat
      901 atcccaatgg catcgtaaag aac
//
