LOCUS       sequence-152492-         828 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  FUGW lentiviral vector with constitutively expressed destabilized 
            GFP (d2GFP) regulated by the human ZEB1 3'UTR. When used in 
            combination with Ecad-RFP, comprises the Z-cad dual sensor..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 828)
  AUTHORS   Toneff MJ, Sreekumar A, Tinnirello A, Hollander PD, Habib S, Li S, 
            Ellis MJ, Xin L, Mani SA, Rosen JM
  TITLE     The Z-cad dual fluorescent sensor detects dynamic changes between 
            the epithelial and mesenchymal cellular states.
  JOURNAL   BMC Biol. 2016 Jun 17;14(1):47.
  PUBMED    27317311
REFERENCE   2  (bases 1 to 828)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Biol.";
            date: "2016-06-17"; volume: "14(1)"; pages: "47"
COMMENT     Sequence Label: FUGW-d2GFP-ZEB1 3'UTR
FEATURES             Location/Qualifiers
     source          1..828
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     ncRNA           complement(39..80)
                     /label=3' miR-155
                     /note="sequence downstream of the precursor of mouse 
                     miR-155 microRNA (Uva et al., 2013)"
                     /ncRNA_class="miRNA"
ORIGIN
        1 atccagaggt tgattatcga taagcttgat atcgaattgg ccatttgttc catgtgagtg
       61 ctagtaacag gccttgtgtc ctgctagcct agtacagcag ttcaggcttg ttgatgcatt
      121 ttattgtgag atgggagtct ggtaaaaaac tagaaaatga ccatgatgta acaaggaact
      181 taaaaatgaa gagtcagatc tgtaacttac atacatgaat acaggaacaa attggcacaa
      241 aatgttaaac attgttccca acactgttta tagaagttaa tcctaatttg gcttactaag
      301 gaaatgagag ttgtatggtt aggctgattt cttaataaaa ctgcagagca tcatgctatt
      361 agtcacacag tgaatttcaa aatttaagtt ttatattaaa aactgggtaa aggtaaaatg
      421 acttgattgt agttgtaaaa ctaatacatt cccatttaag ttctgttcta cagtccaagg
      481 caagtataaa tgttaacata acactgttaa atcaaatctc aatgcaggag aaccaattgc
      541 atctctactt taaatcttat caactttcca aattttcata ctaaaatata ttattgtatt
      601 aatacaaact acagtattat acactacact gtgtaataaa taaagaaata taaaaataag
      661 acacataaat ataaaagttt tctaaaacta aaagtacata tgtcagtaag aagggtatta
      721 atactgccag gtttgaagac atacagtaca aaaatgttgc acagatctat aaactaaaag
      781 aaataaaata atactgatag gtaaaaatca gctaatgttg ttaataaa
//
