LOCUS       sequence-152493-         843 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pHAGE lentiviral vector with dsRed driven by the human E-cadherin 
            promoter. When used in combination with FUGW-d2GFP-ZEB1 3' UTR, 
            comprises the Z-cad dual sensor. Use 2nd generation packaging 
            system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 843)
  AUTHORS   Toneff MJ, Sreekumar A, Tinnirello A, Hollander PD, Habib S, Li S, 
            Ellis MJ, Xin L, Mani SA, Rosen JM
  TITLE     The Z-cad dual fluorescent sensor detects dynamic changes between 
            the epithelial and mesenchymal cellular states.
  JOURNAL   BMC Biol. 2016 Jun 17;14(1):47.
  PUBMED    27317311
REFERENCE   2  (bases 1 to 843)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Biol.";
            date: "2016-06-17"; volume: "14(1)"; pages: "47"
COMMENT     Sequence Label: pHAGE-E-cadherin-RFP
FEATURES             Location/Qualifiers
     source          1..843
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      138..147
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 cttcagcttg gcggtctggg tgccctcgta ggggcggccc tcgccctcgc cctcgatctc
       61 gaactcgtgg ccgttcacgg agccctccat gcgcaccttg aagcgcatga actccttgat
      121 gacgtcctcg gaggaggcca tggcggccgc ggctggccgg ggacgccgag cgagggcagg
      181 cgccgggtgc ggtcgggtcg ggccgggctg gagcgggctg gagtctgaac tgacttccgc
      241 aagctcacag gtgctttgca gttccgacgc cactgagagg gggtgcgtgg ctgcagccag
      301 gtgagccccg gaggcaccgc cccccgtacc gctgattggc tgagggttca cctgccggcc
      361 acagccaatc agcagcgcgg acccctcccc agggcggagc tgacggcccg cccacccggc
      421 ctcgcataga cgcgttgacc ctctagcctg gagttgctag ggtctaggtg ggttatggga
      481 cctgcacggt tctgattcca ctgggttcga atcccagccc cgccggtggc tcactaagac
      541 ctgggatcag aaagggcttt tacacttggc tgagttcttt tgttttttgg gattttttgt
      601 ttgttcgttt tggagatggg gctcactctt tcacccaagc tggagtgcag tggtgcgatc
      661 acagctcact gcagcctcga actcctgggc tcaagcggtc ctctggcccc agcctctcta
      721 gtagctggga gtacaggtgc acaccaccac gccaggctaa tttttttttt cttttgcctt
      781 tttgtagaga gacaagtcgg ggcggacagg ggcagtgggg gcgctgtgtc tccctgtatt
      841 gcc
//
