LOCUS       sequence-152600-         695 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway cloning compatible binary vector for C-terminal fusion with 
            GUS (no promoter)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 695)
  AUTHORS   Nakagawa T, Suzuki T, Murata S, Nakamura S, Hino T, Maeo K, Tabata 
            R, Kawai T, Tanaka K, Niwa Y, Watanabe Y, Nakamura K, Kimura T, 
            Ishiguro S
  TITLE     Improved Gateway binary vectors: high-performance vectors for 
            creation of fusion constructs in transgenic analysis of plants.
  JOURNAL   Biosci Biotechnol Biochem. 2007 Aug;71(8):2095-100. Epub 2007 Aug 7.
  PUBMED    17690442
REFERENCE   2  (bases 1 to 695)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biosci 
            Biotechnol Biochem."; date: "2007-08"; volume: "71(8)"; pages: 
            "2095-100. Epub 2007 Aug 7"
COMMENT     Sequence Label: pGWB433
FEATURES             Location/Qualifiers
     source          1..695
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(join(84..90,91..108,109..114))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(151..275)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(316..333)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(316..332)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(325..347)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    535..559
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
ORIGIN
        1 ggtggtatat ccagtgattt ttttctccat tttagcttcc ttagctcctg aaaatctcga
       61 cggatcctaa ctcaaaatcc acacattata cgagccggaa gcataaagtg taaagcctgg
      121 ggtgcctaat gcggcccctc gaggggccgc catagtgact ggatatgttg tgttttacag
      181 tattatgtag tctgtttttt atgcaaaatc taatttaata tattgatatt tatatcattt
      241 tacgtttctc gttcagcttt tttgtacaaa cttgttgata actctagagt cgacctgcag
      301 gcatgcaagc ttggcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt
      361 tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga
      421 ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat gctagagcag
      481 cttgagcttg gatcagattg tcgtttcccg ccttcagttt aaactatcag tgtttgacag
      541 gatatattgg cgggtaaacc taagagaaaa gagcgtttat tagaataatc ggatatttaa
      601 aagggcgtga aaaggtttat ccgttcgtcc atttgtatgt gcatgccaac cacagggttc
      661 ccctcgggat caaagtactt tgatccaacc cctcc
//
