LOCUS       sequence-152630-         608 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway cloning compatible binary vector for C-terminal fusion with 
            eYFP (CaMV35S promoter)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 608)
  AUTHORS   Nakagawa T, Suzuki T, Murata S, Nakamura S, Hino T, Maeo K, Tabata 
            R, Kawai T, Tanaka K, Niwa Y, Watanabe Y, Nakamura K, Kimura T, 
            Ishiguro S
  TITLE     Improved Gateway binary vectors: high-performance vectors for 
            creation of fusion constructs in transgenic analysis of plants.
  JOURNAL   Biosci Biotechnol Biochem. 2007 Aug;71(8):2095-100. Epub 2007 Aug 7.
  PUBMED    17690442
REFERENCE   2  (bases 1 to 608)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biosci 
            Biotechnol Biochem."; date: "2007-08"; volume: "71(8)"; pages: 
            "2095-100. Epub 2007 Aug 7"
COMMENT     Sequence Label: pGWB541
FEATURES             Location/Qualifiers
     source          1..608
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(join(84..90,91..108,109..114))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(151..275)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        complement(299..345)
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     primer_bind     complement(334..354)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
ORIGIN
        1 ggtggtatat ccagtgattt ttttctccat tttagcttcc ttagctcctg aaaatctcga
       61 cggatcctaa ctcaaaatcc acacattata cgagccggaa gcataaagtg taaagcctgg
      121 ggtgcctaat gcggcccctc gaggggccgc catagtgact ggatatgttg tgttttacag
      181 tattatgtag tctgtttttt atgcaaaatc taatttaata tattgatatt tatatcattt
      241 tacgtttctc gttcagcttt tttgtacaaa cttgttgata actctagagt cccccgtgtt
      301 ctctccaaat gaaatgaact tccttatata gaggaagggt cttgcgaagg atagtgggat
      361 tgtgcgtcat cccttacgtc agtggagata tcacatcaat ccacttgctt tgaagacgtg
      421 gttggaacgt cttctttttc cacgatgctc ctcgtgggtg ggggtccatc tttgggacca
      481 ctgtcggcag aggcatcttc aacgatggcc tttcctttat cgcaatgatg gcatttgtag
      541 gagccacctt ccttttccac tatcttcaca ataaagtgac agatagctgg gcaatggaat
      601 ccgaggag
//
