LOCUS       sequence-152852-        1051 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  TOPO construct expressing UBC for generation of GMAP-compatible 
            promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1051)
  AUTHORS   Akama-Garren EH, Joshi NS, Tammela T, Chang GP, Wagner BL, Lee DY, 
            Rideout Iii WM, Papagiannakopoulos T, Xue W, Jacks T
  TITLE     A Modular Assembly Platform for Rapid Generation of DNA Constructs.
  JOURNAL   Sci Rep. 2016 Feb 18;6:16836. doi: 10.1038/srep16836.
  PUBMED    26887506
REFERENCE   2  (bases 1 to 1051)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2016-02-18"; volume: "6:16836. doi"; pages: " 
            10.1038/srep16836"
COMMENT     Sequence Label: TOPO UBC
FEATURES             Location/Qualifiers
     source          1..1051
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        14..32
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     14..31
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     promoter        105..504
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
ORIGIN
        1 attacgccaa gctatttagg tgacactata gaatactcaa gctatgcatc aagcttggta
       61 ccgagctcgg atcagtgtga gggagtgtaa agctggtttg atctggcctc cgcgccgggt
      121 tttggcgcct cccgcgggcg cccccctcct cacggcgagc gctgccacgt cagacgaagg
      181 gcgcagcgag cgtcctgatc cttccgcccg gacgctcagg acagcggccc gctgctcata
      241 agactcggcc ttagaacccc agtatcagca gaaggacatt ttaggacggg acttgggtga
      301 ctctagggca ctggttttct ttccagagag cggaacaggc gaggaaaagt agtcccttct
      361 cggcgattct gcggagggat ctccgtgggg cggtgaacgc cgatgattat ataaggacgc
      421 gccgggtgtg gcacagctag ttccgtcgca gccgggattt gggtcgcggt tcttgtttgt
      481 ggatcgctgt gatcgtcact tggtgagtag cgggctgctg ggctggccgg ggctttcgtg
      541 gccgccgggc cgctcggtgg gacggaagcg tgtggagaga ccgccaaggg ctgtagtctg
      601 ggtccgcgag caaggttgcc ctgaactggg ggttgggggg agcgcagcaa aatggcggct
      661 gttcccgagt cttgaatgga agacgcttgt gaggcgggct gtgaggtcgt tgaaacaagg
      721 tggggggcat ggtgggcggc aagaacccaa ggtcttgagg ccttcgctaa tgcgggaaag
      781 ctcttattcg ggtgagatgg gctggggcac catctgggga ccctgacgtg aagtttgtca
      841 ctgactggag aactcggttt gtcgtctgtt gcgggggcgg cagttatggc ggtgccgttg
      901 ggcagtgcac ccgtaccttt gggagcgcgc gccctcgtcg tgtcgtgacg tcacccgttc
      961 tgttggctta taatgcaggg tggggccacc tgccggtagg tgtgcggtag gcttttctcc
     1021 gtcgcaggac gcagggttcg ggcctagggt a
//
