LOCUS       sequence-152879-         842 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  For generating adeno-associated virus to express GFE3 under the EF1a
            promoter in a 'Cre-on' dependent manner..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 842)
  AUTHORS   Gross GG, Straub C, Perez-Sanchez J, Dempsey WP, Junge JA, Roberts 
            RW, Trinh LA, Fraser SE, De Koninck Y, De Koninck P, Sabatini BL, 
            Arnold DB
  TITLE     An E3-ligase-based method for ablating inhibitory synapses.
  JOURNAL   Nat Methods. 2016 Jun 6. doi: 10.1038/nmeth.3894.
  PUBMED    27271196
REFERENCE   2  (bases 1 to 842)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2016 Jun 6. doi: 10.1038/nmeth.3894."
COMMENT     Sequence Label: pAAV-Ef1a-DIO_EGFP-GFE3
FEATURES             Location/Qualifiers
     source          1..842
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(38..71)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    complement(122..155)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             complement(355..435)
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
     primer_bind     complement(802..823)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 tgtcgtgagg tccggatcct ctagagtcga ctccggaata acttcgtata ggatacttta
       61 tacgaagtta tgcagaatgg tagctggatt gtagctgcta ttagcaatat gaaacctctt
      121 aataacttcg tatagcatac attatacgaa gttatggcgc gccttacttg tacattaaga
      181 cataaaaatt ttttgcttga acgtaatgac tgtgcagcac atgggacatt tgtcaactgc
      241 ttccgcacac tgtttacaag tgaccagatg tccacaagga acaaaaacta tagcaatatt
      301 tctatccata cagattttgc aaagcttctc ctcttgtagg cgccttaggc tagcagcgta
      361 atctggaaca tcgtatgggt aagcgtaatc tggaacatcg tatgggtaag cgtaatctgg
      421 aacatcgtat gggtaagatc ttgaaccggt gcggtagttg atggagatcg ggatcgggtc
      481 ctggtagttc cagtggtcga tcgtgacggc gtacaccgtg atggtatagt cgacaccagg
      541 tttcaggctg ctgatggtag cagtggactt gctgccaggc acggtgaatt cctggacagg
      601 gctattgcca ccagtttcac cgtaggtgat gcggtagtag cgaaccatga ccttgtactt
      661 gccccagctg atctggatgc tggttggtga tgcttccttg acttcgagca attgcgaacc
      721 tcctcctgat ccacctccag aacctccacc agaacctcca ccgtacagct cgtccatgcc
      781 gagagtgatc ccggcggcgg tcacgaactc cagcaggacc atgtgatcgc gcttctcgtt
      841 gg
//
