LOCUS       sequence-152880-         811 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  For generating adeno-associated virus to express RandE3 under the 
            EF1a promoter in a 'Cre-on' dependent manner..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 811)
  AUTHORS   Gross GG, Straub C, Perez-Sanchez J, Dempsey WP, Junge JA, Roberts 
            RW, Trinh LA, Fraser SE, De Koninck Y, De Koninck P, Sabatini BL, 
            Arnold DB
  TITLE     An E3-ligase-based method for ablating inhibitory synapses.
  JOURNAL   Nat Methods. 2016 Jun 6. doi: 10.1038/nmeth.3894.
  PUBMED    27271196
REFERENCE   2  (bases 1 to 811)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2016 Jun 6. doi: 10.1038/nmeth.3894."
COMMENT     Sequence Label: pAAV-Ef1a-DIO_EGFP-RandE3
FEATURES             Location/Qualifiers
     source          1..811
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(38..71)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    complement(122..155)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             complement(355..435)
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
ORIGIN
        1 gtcgtgaggt accggatcct ctagagtcga ctccggaata acttcgtata ggatacttta
       61 tacgaagtta tgcagaatgg tagctggatt gtagctgcta ttagcaatat gaaacctctt
      121 aataacttcg tatagcatac attatacgaa gttatggcgc gccttacttg tacattaaga
      181 cataaaaatt ttttgcttga acgtaatgac tgtgcagcac atgggacatt tgtcaactgc
      241 ttccgcacac tgtttacaag tgaccagatg tccacaagga acaaaaacta tagcaatatt
      301 tctatccata cagattttgc aaagcttctc ctcttgtagg cgccttaggc tagcagcgta
      361 atctggaaca tcgtatgggt aagcgtaatc tggaacatcg tatgggtaag cgtaatctgg
      421 aacatcgtat gggtaagatc tggtgcggta gttgatggag atcggggact ggtcggagct
      481 ggcctggggc tcgcgcgtga cggcgtacac cgtgatggta tagtcgacac caggtttcag
      541 gccgctgatg gtagcagtgg acttgctgcc aggcacggtg aattcctgga cagggctatt
      601 gccaccagtt tcaccgtagg tgatgcggta gtagcgcgcg tgctcctgcg tcgcccgcca
      661 gctgattagg aggctggtcg gagtcgcaac gacgacttcg agcaattgcg aacctcctcc
      721 tgatccacct ccagaacctc caccagaacc tccaccgtac agctcgtcca tgccgagagt
      781 gatcccggcg gcggtcacga actccagcag g
//
