LOCUS       sequence-152917-        1018 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Sacsin Ubl-sr1 (SIRPT1 repeat) domain (1-339) in fusion 
            with GST (bacterial).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1018)
  AUTHORS   Menade M, Kozlov G, Trempe JF, Pande H, Shenker S, Wickremasinghe S,
            Li X, Hojjat H, Dicaire MJ, Brais B, McPherson PS, Wong MJH, Young 
            JC, Gehring K
  TITLE     Structures of ubiquitin-like (Ubl) and Hsp90-like domains of sacsin 
            provide insight into pathological mutations.
  JOURNAL   J Biol Chem. 2018 Aug 17;293(33):12832-12842. doi: 
            10.1074/jbc.RA118.003939. Epub 2018 Jun 26.
  PUBMED    29945973
REFERENCE   2  (bases 1 to 1018)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.RA118.003939"; journalName: "J Biol Chem"; date: 
            "2018-08-17- 17"; volume: "293"; issue: "33"; pages: "12832-12842"
COMMENT     Sequence Label: pGEX6P1-Ubl-sr1(SIRPT1)
FEATURES             Location/Qualifiers
     source          1..1018
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             9..32
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
ORIGIN
        1 aatcggatct ggaagttctg ttccaggggc ccctgggatc catggagacc aaggagaaca
       61 ggtgggtccc ggtgaccgtg ctccccggct gcgtgggctg caggaccgtc gcggcgctgg
      121 cgtcctggac cgtgcgcgat gtgaaggaac gtatcttcgc ggagactggc ttcccggtgt
      181 cggagcagcg gctgtggcgc ggcggccgcg agttatctga ctggatcaag attggagatc
      241 tgacttccaa aaattgtcat ctttttgtaa accttcaatc aaaaggctta aaagggggag
      301 gtcgatttgg tcagacaacg ccaccacttg ttgattttct caaggacatt ttgagaagat
      361 atccagaagg aggacagatt cttaaggaat taattcagaa tgcagaagat gctggggcga
      421 cagaagttaa atttttatat gatgaaactc aatacggaac agagactctt tggtcaaaag
      481 atatggcgcc atatcagggg ccagctctct atgtgtacaa caacgcggtt ttcaccccag
      541 aggactggca cggcattcaa gaaatagcaa gaagcaggaa aaaggatgat cctctgaagg
      601 tcggaagatt tggaattggg tttaattctg tctatcatat aacagatgtt ccttgtatct
      661 ttagtggtga ccaaatcggg atgctagatc ctcatcaaac actttttggc ccacatgaat
      721 caggccaatg ttggaatctc aaagatgaca gcaaagaaat tagtgaactt tcagaccagt
      781 ttgcaccatt tgttggcatt tttggaagca ccaaggaaac atttataaac ggcaattttc
      841 caggaacatt tttccgtttc cctcttcgcc tacaaccttc acaacttagt agtaacctct
      901 acaataagca gaaggttctt gagttgtttg agtcttttag ggcagatgcg gacacagtgc
      961 tgctctttct gaaaagtgtg caggatgttt ccttatatgt ccgagaggct gacggaac
//
