LOCUS       sequence-153003-         998 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pET-Duet for bacterial expression of Cys-NuG2(4x)-Ald and 
            co-expression of Mycobacterium tuberculosis Formylglycine Generating
            Enzyme.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 998)
  AUTHORS   Walder R, LeBlanc MA, Van Patten WJ, Edwards D, Greenberg JA, 
            Adhikari A, Okoniewski SR, Sullan RMA, Rabuka D, Sousa MC, Perkins 
            TT
  TITLE     Rapid Characterization of a Mechanically Labile alpha-helical 
            Protein Enabled by Efficient Site-Specific Bioconjugation.
  JOURNAL   J Am Chem Soc. 2017 Jul 5. doi: 10.1021/jacs.7b02958.
  PUBMED    28677396
REFERENCE   2  (bases 1 to 998)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Am Chem 
            Soc. 2017 Jul 5. doi: 10.1021/jacs.7b02958."
COMMENT     Sequence Label: pMS1088
FEATURES             Location/Qualifiers
     source          1..998
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(36..53)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 gcttcctttc gggctttgtt agcagccgga tctcagtggt ggtggtggtg gtgctcgagc
       61 tacccggaca ccgggtcggc cacgcaccgg aacccgatat gggtggtcgc ggtgtcctgc
      121 gactgcggcg agcgcgccgc cgggcggtag cggtggcagt actccggcgc gcacaggtgc
      181 gagccgccct tgagggtctg gctgatcgtc gggtcggcgg ctgtagcgag cttgaccggt
      241 gcgcagcagg ccgtcgaggg tggatcgatg cgatggtgtg gatagaactc ggtggtggtc
      301 cactcccaaa cgtttccgat catgtcgagc aagccaaacc cgttggccgg aaacctgccc
      361 accggggagg ttcccaccca gcccaatgca ccgtcgttgc ggtaaggaaa ccggccctgc
      421 caggtgttcg ccatgagcat gccccccggc ttctcctggt cgccccacgc ataggttgcc
      481 gtggttccgc cacgggccgc gtactcccac tcggcctcgg tcggtaggcg tcgaccagcc
      541 catcgtgcgt aggccacggc gtccggatag gccacctgta cgaccgggtg gccggctcgg
      601 tcggcgatat cgctgtcccg gccaaacgga tggcgccagc aggcgccagg tacccagtcc
      661 caccattgcc gccagtcacg caggtcgacc ggcccggccg tcggacaaaa caccatcgca
      721 ccgggacaca ggtctgctgc gtccactcct gggtagagcc cggggtcaag gggttgttct
      781 gcaaccgtca catagcctgt cgcggagacg aattcggcaa attgcgcgtt ggtcaccggg
      841 tgtcgctcta ccgcaaaggc gcgcacggtc acggtatgaa tcggcgcttc ttcggggtag
      901 aagcgcgtcg agcccatgcg gaacgatccg ccgggcaggt caaccaactc ggtcagcata
      961 tctatatctc cttcttatac ttaactaata tactaaga
//
