LOCUS       sequence-153033-        1097 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  N-terminal tagging of human FANCF with 3xFLAG_Twin_Strep.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1097)
  AUTHORS   Dalvai M, Loehr J, Jacquet K, Huard CC, Roques C, Herst P, Cote J, 
            Doyon Y
  TITLE     A Scalable Genome-Editing-Based Approach for Mapping Multiprotein 
            Complexes in Human Cells.
  JOURNAL   Cell Rep. 2015 Oct 7. pii: S2211-1247(15)01020-7. doi: 
            10.1016/j.celrep.2015.09.009.
  PUBMED    26456817
REFERENCE   2  (bases 1 to 1097)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep. 
            2015 Oct 7. pii: S2211-1247(15)01020-7. doi: 
            10.1016/j.celrep.2015.09.009."
COMMENT     Sequence Label: FancF_N_FS_v2_Donor
FEATURES             Location/Qualifiers
     source          1..1097
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(join(777..800,801..836,837..860,861..866))
                     /codon_start=1
                     /product="two Strep-Tag(R)II moieties connected by a linker
                     for enhanced binding to Strep-Tactin(R), an engineered form
                     of streptavidin (Schmidt et al., 2013)"
                     /label=Twin-Strep-tag
                     /translation="SAWSHPQFEKGGGSGGGSGGSAWSHPQFEK"
     CDS             complement(876..941)
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     regulatory      953..962
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 aaccgcctct ccccgcgcgt tggccgattc attaatgcag aattccccag aagccagtgg
       61 actagcactt ggctcccctc tccaggtgat ttgtggatgc cgggttccaa ctcttcttgg
      121 ggccgacgag acaaaggcgg ctgcaacagc gccaccgcta tcaccttcag gaagttgttc
      181 tgaggcaagc gctcccacag gctgctgaga aacctggcgg gacgctccgc ttcggccttc
      241 cccacctcct gcagacgctc cagcagcagc tccgcctggg tcttcatcag agagtcctcc
      301 tggagatttg ggttctctct atagccattg aagcgcagca tgtgcaccgc agaccgccgg
      361 cgggcaaggc gggccaggct ctcttggagt gtctcctcat cggcgtcccg gacgcccggg
      421 ccgggaaaga gttgctgcac caggtggtaa cgagctgcat ccccgagggc ccggttctcc
      481 agcaggcgca gagagagcag gacgtcacag tgaccgaggg cctggaagtt cgctaatccc
      541 ggaactggac cccgcccaaa gccgccctct tgcctccact ggttgtgcag ccgccgctcc
      601 agagccgtgc gaatggggcc atgccgacca aagcgccgat ggatgtggcg caggtagcgc
      661 gcccactgca aggcccggcg cacggtggcg gggtcccagg tgctgacgta ggtagtgctt
      721 gagaccgcca gaagctcgga aaagcgatcc aggtgctgca gaagggattc ggcgcccttt
      781 tcgaactggg ggtggctcca agcagatcct ccgcttccgc cgccagagcc tccgcccttc
      841 tcaaactgag ggtgggacca ggcgctgctg gcgcccttgt catcgtcgtc tttgtagtcg
      901 atgtcgtgat ccttataatc gccgtcgtgg tccttgtagt cgccggcgga tcccatggtg
      961 gcgaggtgcg cgaaggccct acttccgctt tcaccttgga gacggcgact ctctgcgtac
     1021 tgattggaac atccgcgaaa tgatacgcct ctctgcaatg ctattggtcg aaatgcatgt
     1081 caatctccca gcgtctt
//
