LOCUS       sequence-153070-         995 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses EGFP-tagged DmCaf40 in S2 cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 995)
  AUTHORS   Bawankar P, Loh B, Wohlbold L, Schmidt S, Izaurralde E
  TITLE     NOT10 and C2orf29/NOT11 form a conserved module of the CCR4-NOT 
            complex that docks onto the NOT1 N-terminal domain.
  JOURNAL   RNA Biol. 2013 Feb;10(2):228-44. doi: 10.4161/rna.23018. Epub 2013 
            Jan 9.
  PUBMED    23303381
REFERENCE   2  (bases 1 to 995)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.4161/rna.23018";
            journalName: "RNA Biol"; date: "2013-02"; volume: "10"; issue: "2"; 
            pages: "228-44"
COMMENT     Sequence Label: pAc5.1B-EGFP-DmCaf40
FEATURES             Location/Qualifiers
     source          1..995
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     38..55
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     CDS             complement(78..95)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(105..146)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 ggatcctcga tcgaggctga tcagcgagct caggccttag aaggcacagt cgaggctgat
       61 cagcgggttt aaactcaatg gtgatggtga tgatgaccgg tacgcgtaga atcgagaccg
      121 aggagagggt tagggatagg cttaccttcg aaccgcgggc cctctagact aggagcccag
      181 tggcgacatg ccgatctgct gcggagtggc gccgagttcc aggttcttga gcagcatctg
      241 cagccactgc ttggtcgact tgtcctcttg caggcacagc gcaaaggtgc cgtcacgcag
      301 ctgatcgggc agacactgtc ccagggcctt gcgagcgcgt gtattgtccg aaagacgcag
      361 atagcagcgc accacatgct tcaacagccg ggcgcatgga tccttcgcca gctggatgac
      421 cattttgccc agggtgatgg ccacgtgcga aaagcgttcg taggtctggc agatgtacga
      481 cagacccgac tcatcgagca ggatcttctg gatgatgaaa gtggccacag tcttgctcag
      541 ctccgatccg ctgtccatga tgctcagaca aaggggcacg atctcggtgg tcagcagaaa
      601 ggtgatcacc tcctgttcgt cggtcttgac cagagcacca atcacgccca aactggtcag
      661 gcgcaagtac tcgaagggcc tggtcttgga cgtggtcgac aagaaagggt acaagtacag
      721 cggtatctgg gcctgcagga aggccgtgcg agtctccggg tgcgaggcga cgcactgcag
      781 cagcgccagg gcgttgcaca cgcggttcga ctggtgggcc gtcaaagtgg gcggcgttat
      841 cgatgggtag atgttaacaa tctcctgcaa cagggcgcag gcggtcccga agctgttcca
      901 cagcatgggg gccaggtccg tctcacgctt cttgcttagc tcgagcagag cggtttcacg
      961 cgtgtccgga tgggccagct cattgatcca ctggt
//
