LOCUS       sequence-153072-         990 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses EGFP-tagged DmNot11 in S2 cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 990)
  AUTHORS   Bawankar P, Loh B, Wohlbold L, Schmidt S, Izaurralde E
  TITLE     NOT10 and C2orf29/NOT11 form a conserved module of the CCR4-NOT 
            complex that docks onto the NOT1 N-terminal domain.
  JOURNAL   RNA Biol. 2013 Feb;10(2):228-44. doi: 10.4161/rna.23018. Epub 2013 
            Jan 9.
  PUBMED    23303381
REFERENCE   2  (bases 1 to 990)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.4161/rna.23018";
            journalName: "RNA Biol"; date: "2013-02"; volume: "10"; issue: "2"; 
            pages: "228-44"
COMMENT     Sequence Label: pAc5.1B-EGFP-DmNot11
FEATURES             Location/Qualifiers
     source          1..990
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             742..783
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             793..810
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(833..850)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     primer_bind     complement(907..926)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     961..980
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 gcatggacga gctgtacaag ccaagcttaa ttaagatgtt tctgtccatc gccccgcccc
       61 taatggactt cgaggacgag ctgctctgga taaaccagtc gaacagcgac aaggtggaga
      121 tcatctacga caagtccaac tacattaccc ccaacataaa gcacctcatc gagcaggctt
      181 ttctccagcc actcagcctc cagaaccagc acatcctctt cgacgacctg ctgaagcaga
      241 acacggacat tgcgcgccaa tatggactga ccccggacaa gctacctctg ctcgtggaaa
      301 gcaatcctct catctccatt gagatcttgc taagactgat gacgacctcc gacataactg
      361 aatacttcaa cgtgttggtc aacatggaca taacacttca ctccatggag gtggtcaacc
      421 gactaaccac ctccggacct ttgcccactg agtttattca cctatacatc agcaattgca
      481 tctcgacttg cgaaacagtc aaggacaagt acatgcagtc tcgcctggtg cgactcgttt
      541 gcgtatttct gcagtccttg attaggaaca agattgtgaa cgttagggaa ctattcattg
      601 aaatcgaagc cttctgcgtg ggtttcagca aaatcaagga ggctgctacc ctgtaccggc
      661 ttatcaaaca cttggaaact ggggaaagcg ctctgtcgtc caactcccta gctaagtagt
      721 ctagagggcc cgcggttcga aggtaagcct atccctaacc ctctcctcgg tctcgattct
      781 acgcgtaccg gtcatcatca ccatcaccat tgagtttaaa cccgctgatc agcctcgact
      841 gtgccttcta aggcctgagc tcgctgatca gcctcgatcg aggatccaga catgataaga
      901 tacattgatg agtttggaca aaccacaact agaatgcagt gaaaaaaatg ctttatttgt
      961 gaaatttgtg atgctattgc tttatttgta
//
