LOCUS       sequence-153075-         758 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Base vector for construction of series of suicide plasmids for gene 
            replacement in Proteobacteria.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 758)
  AUTHORS   Quandt J, Hynes MF
  TITLE     Versatile suicide vectors which allow direct selection for gene 
            replacement in gram-negative bacteria.
  JOURNAL   Gene. 1993 May 15;127(1):15-21.
  PUBMED    8486283
REFERENCE   2  (bases 1 to 758)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Gene."; 
            date: "1993-05-15"; volume: "127(1)"; pages: "15-21"
COMMENT     Sequence Label: pJQ200
FEATURES             Location/Qualifiers
     source          1..758
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 ctgctgtaca ttttgatacg tttttccgtc accgtcaaag attgatttat aatcctctac
       61 accgttgatg ttcaaagagc tgtctgatgc tgatacgtta acttgtgcag ttgtcagtgt
      121 ttgtttgccg taatgtttac cggagaaatc agtgtagaat aaacggattt ttccgtcaga
      181 tgtaaatgtg gctgaacctg accattcttg tgtttggtct tttaggatag aatcatttgc
      241 atcgaatttg tcgctgtctt taaagacgcg gccagcgttt ttccagctgt caatagaagt
      301 ttcgccgact ttttgataga acatgtaaat cgatgtgtca tccgcatttt taggatctcc
      361 ggctaatgca aagacgatgt ggtagccgtg atagtttgcg acagtgccgt cagcgttttg
      421 taatggccag ctgtcccaaa cgtccaggcc ttttgcagaa gagatatttt taattgtgga
      481 cgaatcgaat tcaggaactt gatatttttc atttttttgc tgttcaggga tttgcagcat
      541 atcatggcgt gtaatatggg aaatgccgta tgtttcctta tatggctttt ggttcgtttc
      601 tttcgcaaac gcttgagttg cgcctcctgc cagcagtgcg gtagtaaagg ttaatactgt
      661 tgcttgtttt gcaaactttt tgatgttcat cgttcatgtc tcctttttta tgtactgtgt
      721 tagcggtctg cttcttccag ccctcctgtt tgaagatg
//
