LOCUS       sequence-153141-         869 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid was constructed to study cortactin interactions.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 869)
  AUTHORS   Weed SA, Karginov AV, Schafer DA, Weaver AM, Kinley AW, Cooper JA, 
            Parsons JT
  TITLE     Cortactin localization to sites of actin assembly in lamellipodia 
            requires interactions with F-actin and the Arp2/3 complex.
  JOURNAL   J Cell Biol. 2000 Oct 2;151(1):29-40.
  PUBMED    11018051
REFERENCE   2  (bases 1 to 869)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol."; date: "2000-10-2"; volume: "151(1)"; pages: "29-40"
COMMENT     Sequence Label: pcDNA3-Flag-Cortactin
FEATURES             Location/Qualifiers
     source          1..869
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     54..73
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        54..72
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      85..94
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             94..117
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 agcagagctc tctggctaac tagagaaccc actgcttact ggcttatcga aattaatacg
       61 actcactata gggagaccca agctgccacc atggactaca aggacgacga tgacaaggga
      121 cctaagcttg gtaccatgtg gaaagcctct gcaggccatg ctgtgtccat cacgcaggat
      181 gatggaggag ctgatgactg ggagactgat cctgattttg tgaatgatgt gagtgaaaag
      241 gagcagagat ggggtgctaa aaccgtgcag ggatcggggc accaggaaca catcaacatt
      301 cacaagcttc gagagaatgt cttccaagaa caccagacgc tcaaggagaa ggagctggaa
      361 acgggaccca aggcttccca cggctatggc gggaagttcg gtgtggagca ggataggatg
      421 gacagatcag ccgtgggcca tgagtaccag tcgaagcttt ccaagcactg ctcacaagtg
      481 gactcggtcc ggggcttcgg aggcaagttc ggtgtccaga tggacagggt ggatcagtct
      541 gctgtaggct ttgaatacca ggggaagact gagaagcatg cctcccagaa agactactct
      601 agtggcttcg gtggcaaata cggtgtgcaa gctgaccgtg tagacaagag tgccgtgggc
      661 tttgactacc agggcaagac ggagaagcat gagtctcaga aagattactc caaaggtttt
      721 ggtggcaaat atgggattga caaggacaag gtggataaaa gtgctgtggg ctttgagtat
      781 caaggcaaga cagagaagca cgaatcccag aaagactatg taaaaggctt tggaggaaag
      841 tttggtgtgc agacagacag acaggacaa
//
