LOCUS       sequence-153183-        1006 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses lambdaN-HA-tagged human 4E-BP1 in Mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1006)
  AUTHORS   Peter D, Igreja C, Weber R, Wohlbold L, Weiler C, Ebertsch L, 
            Weichenrieder O, Izaurralde E
  TITLE     Molecular architecture of 4E-BP translational inhibitors bound to 
            eIF4E.
  JOURNAL   Mol Cell. 2015 Mar 19;57(6):1074-87. doi: 
            10.1016/j.molcel.2015.01.017. Epub 2015 Feb 19.
  PUBMED    25702871
REFERENCE   2  (bases 1 to 1006)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2015.01.017"; journalName: "Mol Cell"; date: 
            "2015-03-19- 19"; volume: "57"; issue: "6"; pages: "1074-87"
COMMENT     Sequence Label: pLNHA-C1-Hs4E-BP1
FEATURES             Location/Qualifiers
     source          1..1006
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(518..544)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(569..634)
                     /codon_start=1
                     /product="N-terminal RNA-binding domain from the lambda 
                     bacteriophage antiterminator protein N (Baron-Benhamou et 
                     al., 2004)"
                     /label=lambda N peptide
                     /translation="MDAQTRRRERRAEKQAQWKAAN"
     regulatory      631..640
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(667..686)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(668..686)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(731..934)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(760..780)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 ctgcaataaa caagttaaca acaacaattg cattcatttt atgtttcagg ttcaggggga
       61 ggtgtgggag gttttttaaa gcaagtaaaa cctctacaaa tgtggtatgg ctgattatga
      121 tcagttatct agatccggtg gatccttaaa tgtccatctc aaactgtgac tcttcaccgc
      181 ccgcccgctt atcttctggg ctattgcgca ggtggctctg gctggcttcc atggggggct
      241 catcactgga agggctggtg acccccggaa tggtgggcag atcccttggg ggtgttttgg
      301 tcacaggtga gttccgacac tccatcagga atttccggtc atagatgatc ctggtacctc
      361 ccggggtggt gctgaagagc gtgccgccgg gggtcgtgct gtagtccccg ggcgggagct
      421 gcacgccgtc gccgagcacc acccggcgag tggcggggat ggcccggctt ggggtctggc
      481 tgcagctgct gcccccggac atagctcgag atggaggagc gtaatctgga acatcgtatg
      541 ggtaagccat atgtggagga gatccaccgt ttgcagcttt ccattgagct tgtttctcag
      601 cgcgacgctc acgtcgtcgt gtttgtgcgt ccatggtggc gaccggtagc gctagagctt
      661 gggtctccct atagtgagtc gtattaattt cgataagcca gtaagcagtg ggttctctag
      721 ttagccagag agctctgctt atatagacct cccaccgtac acgcctaccg cccatttgcg
      781 tcaatggggc ggagttgtta cgacattttg gaaagtcccg ttgattttgg tgccaaaaca
      841 aactcccatt gacgtcaatg gggtggagac ttggaaatcc ccgtgagtca aaccgctatc
      901 cacgcccatt gatgtactgc caaaaccgca tcaccatggt aatagcgatg actaatacgt
      961 agatgtactg ccaagtagga aagtcccata aggtcatgta ctgggc
//
