LOCUS       sequence-153231-         968 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  retroviral expression of p27 and mCherry.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 968)
  AUTHORS   Kueh HY, Champhekar A, Nutt SL, Elowitz MB, Rothenberg EV
  TITLE     Positive feedback between PU.1 and the cell cycle controls myeloid 
            differentiation.
  JOURNAL   Science. 2013 Aug 9;341(6146):670-3. doi: 10.1126/science.1240831. 
            Epub 2013 Jul 18.
  PUBMED    23868921
REFERENCE   2  (bases 1 to 968)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.1240831"; journalName: "Science"; date: "2013-08-9-
            9"; volume: "341"; issue: "6146"; pages: "670-3"
COMMENT     Sequence Label: p27-IRES-mCherry
FEATURES             Location/Qualifiers
     source          1..968
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      162..171
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             168..878
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(313..331)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     581..600
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 ccagaaggta ccccattgta tgggatctga tctggggcct cggtacacat gctttacatg
       61 tgtttagtcg aggttaaaaa aacgtctagg ccccccgaac cacggggacg tggttttcct
      121 ttgaagaaca cgatgataat atggccacaa ccatgactag tgccaccatg gtgagcaagg
      181 gcgaggagga taacatggcc atcatcaagg agttcatgcg cttcaaggtg cacatggagg
      241 gctccgtgaa cggccacgag ttcgagatcg agggcgaggg cgagggccgc ccctacgagg
      301 gcacccagac cgccaagctg aaggtgacca agggtggccc cctgcccttc gcctgggaca
      361 tcctgtcccc tcagttcatg tacggctcca aggcctacgt gaagcacccc gccgacatcc
      421 ccgactactt gaagctgtcc ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg
      481 aggacggcgg cgtggtgacc gtgacccagg actcctccct gcaggacggc gagttcatct
      541 acaaggtgaa gctgcgcggc accaacttcc cctccgacgg ccccgtaatg cagaagaaga
      601 ccatgggctg ggaggcctcc tccgagcgga tgtaccccga ggacggcgcc ctgaagggcg
      661 agatcaagca gaggctgaag ctgaaggacg gcggccacta cgacgctgag gtcaagacca
      721 cctacaaggc caagaagccc gtgcagctgc ccggcgccta caacgtcaac atcaagttgg
      781 acatcacctc ccacaacgag gactacacca tcgtggaaca gtacgaacgc gccgagggcc
      841 gccactccac cggcggcatg gacgagctgt acaagtaagc atcgataaaa taaaagattt
      901 tatttagtct ccagaaaaag gggggaatga aagaccccac ctgtaggttt ggcaagctag
      961 cttaagta
//
