LOCUS       sequence-153238-         867 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses RpBphP1-mCherry-Intersectin1 DHPH domain under CMVd1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 867)
  AUTHORS   Kaberniuk AA, Shemetov AA, Verkhusha VV
  TITLE     A bacterial phytochrome-based optogenetic system controllable with 
            near-infrared light.
  JOURNAL   Nat Methods. 2016 May 9. doi: 10.1038/nmeth.3864.
  PUBMED    27159085
REFERENCE   2  (bases 1 to 867)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2016 May 9. doi: 10.1038/nmeth.3864."
COMMENT     Sequence Label: pKA-144
FEATURES             Location/Qualifiers
     source          1..867
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     34..53
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        34..52
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        58..76
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     58..75
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 ctggtttagt gaaccgtcag atcactagaa gcttaatacg actcactata gggtacgatt
       61 taggtgacac tatagaataa ggagcgatcg ccatggctag cgtggcaggt catgcctctg
      121 gcagccccgc attcgggacc gccgatcttt cgaattgcga acgtgaagag atccacctcg
      181 ccggctcgat ccagccgcat ggcgcgcttc tggtcgtcag cgagccggat catcgcatca
      241 tccaggccag cgccaacgcc gcggaatttc tgaatctcgg aagcgtgctc ggcgttccgc
      301 tcgccgagat cgacggcgat ctgttgatca agatcctgcc gcatctcgat cccaccgccg
      361 aaggcatgcc ggtcgcggtg cgctgccgga tcggcaatcc ctccacggag tacgacggtc
      421 tgatgcatcg gcctccggaa ggcgggctga tcatcgagct cgaacgtgcc ggcccgccga
      481 tcgatctgtc cggcacgctg gcgccggcgc tggagcggat ccgcacggcg ggctcgctgc
      541 gcgcgctgtg cgatgacacc gcgctgctgt ttcagcagtg caccggctac gaccgggtga
      601 tggtgtatcg cttcgacgag cagggccacg gcgaagtgtt ctccgagcgc cacgtgcccg
      661 ggctcgaatc ctatttcggc aaccgctatc cgtcgtcgga cattccgcag atggcgcggc
      721 ggctgtacga gcggcagcgc gtccgcgtgc tggtcgacgt cagctatcag ccggtgccgc
      781 tggagccgcg gctgtcgccg ctgaccgggc gcgatctcga catgtcgggc tgcttcctgc
      841 gctcgatgtc gccgatccat ctgcagt
//
