LOCUS       sequence-153275-        4556 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Contains yeast counter selectable marker, CYH2, and ampicillin 
            resistance cassette, used for making a DNA capture vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4556)
  AUTHORS   Wolfgang MC, Kulasekara BR, Liang X, Boyd D, Wu K, Yang Q, Miyada 
            CG, Lory S
  TITLE     Conservation of genome content and virulence determinants among 
            clinical and environmental isolates of Pseudomonas aeruginosa.
  JOURNAL   Proc Natl Acad Sci U S A. 2003 Jul 8;100(14):8484-9. Epub 2003 Jun 
            18.
  PUBMED    12815109
REFERENCE   2  (bases 1 to 4556)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2003-07-8"; volume: "100(14)"; pages: 
            "8484-9. Epub 2003 Jun 18"
COMMENT     Sequence Label: pLLX8
FEATURES             Location/Qualifiers
     source          1..4556
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(307..324)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(478..1066)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(558..577)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1237..2028,2029..2097))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1860..1879
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2098..2202)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cgatgaacgc caccggacgc atcgcccgac cgagacgcaa tgccgcaacc aactggttgc
       61 cggccgcccc cgcgccatag atagccaccc tgggcaggcc atcctggcgg ttgagaaatg
      121 gtaccgactg cacagcagag taccagtctc ccatgaaata ctggcgcatg gccagacgca
      181 agccgccgat cagcagcatg ctcaaccacc agtagttgaa caccagggaa cgcgcccggg
      241 cctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc
      301 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
      361 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
      421 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
      481 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
      541 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
      601 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
      661 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
      721 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
      781 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
      841 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
      901 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt
      961 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     1021 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     1081 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     1141 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
     1201 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
     1261 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
     1321 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
     1381 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
     1441 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
     1501 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
     1561 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
     1621 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
     1681 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
     1741 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
     1801 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
     1861 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
     1921 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
     1981 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
     2041 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
     2101 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
     2161 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
     2221 gccacctgac gtgcccgggc atgctacgta cctgtttaac tcttctcatt ttatccgttt
     2281 tctttcttca ccgttcctct taagttcttt atttttttca taacagaatg atcacccctt
     2341 tcactttgcc gccaatataa tattaacaca caagaaataa gtatgaggta gtttgctctc
     2401 gaaaaaacca agtaatagtt caaaaaatga aaaaaagaaa acaaatacca aattatgcca
     2461 ccgttacctt acgtttcatg gttaatcatc gtttactgcc gcctatgagc gtaagctaat
     2521 gttataaaga aacaagctat aatattgtta aatatagttg atcaacagca ttgtaatgat
     2581 tacaagagac gaggtggaat gaaccttatg aaatgcgtat tatatataaa ctgtaataag
     2641 agctaagttg aattgaaatc tacgatactt gatgttgaca ttatagcact agttcccagg
     2701 aaaccctttc gaaaaacaca gcaaaaacaa gagtactgta accaatgtaa catctgtaca
     2761 ccagggaccc acacattacc aaaatcaaaa ttatttttct aatgcctgtt atttttccta
     2821 tttttcctct ggcgcgtgaa tagcccgcag agacgcaaac aattttcctc gcagtttttc
     2881 gcttgtttaa tgcgtatttt cccagatagg ttcaaacctt tcatctgtat cccgtatatt
     2941 taagatggcg tttgctttct ccgttgattt ttttccttct tagtgatttt tttgcattaa
     3001 atcccagaac aatcatccaa ctaatcaaga atgccttcca gattcactaa gactagaaag
     3061 cacagaggtc acgtctcagg tatgtagttc catttggaag agggaatgaa agaaccaaga
     3121 cggtgacttt ttttttagtg ttgtgcaacc aatatgtcgt gtgtatatca tggtacagga
     3181 gaatgtcaat cagctaagtg tactcaacat atttctttgt gttttgattg cgaactttgt
     3241 attaccatct cactgttgag acggcttatt tgaggtaata gctcgagtaa atgtactctt
     3301 ccatcgcaaa ctgagcaaaa agaaagtgtg catagccttt gtcatacttc tcctttatta
     3361 taccatgata ttcagaacag tcatactgtc tactcatttt acggctataa aaggtaactt
     3421 tcatttagat tatggaaagc actaattatc gctgtatcaa atggttgtag agagcgcaat
     3481 tatgaaaaag agttaccacg tttcttttgt ttcgataaaa tgtccagttg aaaacctgtt
     3541 ttactaacga tttaaaaatt gtatttcatt acaatatttt ttttgtacag ccggtaaagg
     3601 tcgtatcggt aagcacagaa agcaccccgg tggtagaggt atggccggtg gtcaacatca
     3661 ccacagaatt aacatggata aataccatcc aggttatttc ggtaaggttg gtatgagata
     3721 cttccacaag caacaagctc atttctggaa gccagtcttg aacttggaca aattgtggac
     3781 attgatccca gaagacaaga gagaccaata cttgaaatct gcttctaagg aaactgctcc
     3841 agttattgac actttggcag ccggttacgg taagatcttg ggtaagggta gaattccaaa
     3901 tgttccagtt atcgtcaaag ctagattcgt ctccaagttg gctgaagaaa aaatcagagc
     3961 tgctggtggt gttgttgaat tgatcgctta agcgcatcaa caaaaactct atgtattttc
     4021 caataaatta tatatcttca gtttaatcta attcaacatc tacttctgta ttatttctat
     4081 cacccatttt caccgttttt tcgtcttgtc tattttgaag ttaccagtgg cagatcccaa
     4141 cgcttttatc gcttttttaa aagattttca aaatccatat ataaacatag cgcaactcct
     4201 tgcccggctt gaagtgcggc acgaacttgc cgtcgaggcg taccgactcc ccggtcttgg
     4261 ggttgcgacc gacgcgcggg gcgcggtagt gcaaggaaaa gctgccgaag ccacggatct
     4321 cgatccggtc cccggtcgcc agggcctggg acatttgctc cagcatggtc ttgattgcca
     4381 actcgacatc cttcgcggac agttgcccct gatgggtaac gatccgttcg atcaactccg
     4441 acttggtcat ggttttccct tctttttcaa gcggctagat cagctcaggt ctcttttagc
     4501 atgctgatca gtctttgaac aggtccaaga aggggaaaac gaaaaagggc ggccta
//
