LOCUS       sequence-153277-         786 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  gRNA to knock out expression of UGCG gene. The product of this gene 
            catalyzes the first step in synthesis of glycosphingolipids..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 786)
  AUTHORS   Stolfa G, Mondal N, Zhu Y, Yu X, Buffone A Jr, Neelamegham S
  TITLE     Using CRISPR-Cas9 to quantify the contributions of O-glycans, 
            N-glycans and Glycosphingolipids to human leukocyte-endothelium 
            adhesion.
  JOURNAL   Sci Rep. 2016 Jul 26;6:30392. doi: 10.1038/srep30392.
  PUBMED    27458028
REFERENCE   2  (bases 1 to 786)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2016-07-26"; volume: "6:30392. doi"; pages: " 
            10.1038/srep30392"
COMMENT     Sequence Label: pX330-UGCG-KO
FEATURES             Location/Qualifiers
     source          1..786
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             25..48
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             55..75
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 ggagactaca aggatcatga tattgattac aaagacgatg acgataagat ggccccaaag
       61 aagaagcgga aggtcggtat ccacggagtc ccagcagccg acaagaagta cagcatcggc
      121 ctggacatcg gcaccaactc tgtgggctgg gccgtgatca ccgacgagta caaggtgccc
      181 agcaagaaat tcaaggtgct gggcaacacc gaccggcaca gcatcaagaa gaacctgatc
      241 ggagccctgc tgttcgacag cggcgaaaca gccgaggcca cccggctgaa gagaaccgcc
      301 agaagaagat acaccagacg gaagaaccgg atctgctatc tgcaagagat cttcagcaac
      361 gagatggcca aggtggacga cagcttcttc cacagactgg aagagtcctt cctggtggaa
      421 gaggataaga agcacgagcg gcaccccatc ttcggcaaca tcgtggacga ggtggcctac
      481 cacgagaagt accccaccat ctaccacctg agaaagaaac tggtggacag caccgacaag
      541 gccgacctgc ggctgatcta tctggccctg gcccacatga tcaagttccg gggccacttc
      601 ctgatcgagg gcgacctgaa ccccgacaac agcgacgtgg acaagctgtt catccagctg
      661 gtgcagacct acaaccagct gttcgaggaa aaccccatca acgccagcgg cgtggacgcc
      721 aaggccatcc tgtctgccag actgagcaag agcagacggc tggaaaatct gatcgcccag
      781 ctgccc
//
