LOCUS       sequence-153356-         933 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of HA tagged ubiquitin with only K48, other 
            lysines mutated to arginines.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 933)
  AUTHORS   Lim KL, Chew KC, Tan JM, Wang C, Chung KK, Zhang Y, Tanaka Y, Smith 
            W, Engelender S, Ross CA, Dawson VL, Dawson TM
  TITLE     Parkin mediates nonclassical, proteasomal-independent ubiquitination
            of synphilin-1: implications for Lewy body formation.
  JOURNAL   J Neurosci. 2005 Feb 23. 25(8):2002-9.
  PUBMED    15728840
REFERENCE   2  (bases 1 to 933)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Neurosci.
            2005 Feb 23. 25(8):2002-9."
COMMENT     Sequence Label: pRK5-HA-Ubiquitin-K48
FEATURES             Location/Qualifiers
     source          1..933
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      68..77
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             80..106
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             137..364
                     /codon_start=1
                     /product="human ubiquitin variant containing Lys-48, with 
                     all other lysines mutated to arginine"
                     /label=ubiquitin-K48
                     /translation="MQIFVRTLTGRTITLEVEPSDTIENVRARIQDREGIPPDQQRLIF
                     AGKQLEDGRTLSDYNIQRESTLHLVLRLRGG"
     regulatory      401..410
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    414..548
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(451..470)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     505..524
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     enhancer        617..808
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(640..660)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
ORIGIN
        1 actgcacctc ggttctatcg attgaattcc cgaaccgaca gtcggtctct tcaccaaggc
       61 cattcgcgcc accatgggct acccctatga tgtgcctgac tacgcagatc tcaatggtgg
      121 tggtggtggg tcgaccatgc agatcttcgt cagaacgtta accggtagaa ccataactct
      181 agaagttgaa ccatccgata ccatcgaaaa cgttagagct agaattcaag acagagaagg
      241 cattccacct gatcaacaaa gattgatctt tgccggtaag cagctcgagg acggtagaac
      301 gctgtctgat tacaacattc agagagagtc caccctgcac ctggtcctcc gtctcagagg
      361 tggttgagcg gccgctaagt aagtaaggat ccccagcttg gccgccatgg cccaacttgt
      421 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
      481 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
      541 tctggatcgg gaattaattc ggcgcagcac catggcctga aataacctct gaaagaggaa
      601 cttggttagg taccttctga ggcggaaaga accagctgtg gaatgtgtgt cagttagggt
      661 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt
      721 cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
      781 atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc
      841 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg
      901 ccgaggccgc ctcggcctct gagctattcc aga
//
