LOCUS       sequence-153389-         543 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of LOV2 anchored to mitochondria.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 543)
  AUTHORS   Wang H, Vilela M, Winkler A, Tarnawski M, Schlichting I, Yumerefendi
            H, Kuhlman B, Liu R, Danuser G, Hahn KM
  TITLE     LOVTRAP: an optogenetic system for photoinduced protein 
            dissociation.
  JOURNAL   Nat Methods. 2016 Jul 18. doi: 10.1038/nmeth.3926.
  PUBMED    27427858
REFERENCE   2  (bases 1 to 543)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2016 Jul 18. doi: 10.1038/nmeth.3926."
COMMENT     Sequence Label: pTriEx-NTOM20-LOV2
FEATURES             Location/Qualifiers
     source          1..543
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             112..540
                     /codon_start=1
                     /gene="NPH1 from Avena sativa"
                     /product="light, oxygen, or voltage domain from phototropin
                     1 of Avena sativa (Herrou and Crosson, 2011)"
                     /label=LOV2
                     /note="photoswitch that undergoes a light-inducible 
                     conformational change"
                     /translation="LATTLERIEKNFVITDPRLPDNPIIFASDSFLQLTEYSREEILGR
                     NCRFLQGPETDRATVRKIRDAIDNQTEVTVQLINYTKSGKKFWNLFHLQPMRDQKGDVQ
                     YFIGVQLDGTEHVRDAAEREGVMLIKKTAENIDEAAKEL"
ORIGIN
        1 atggtgggcc gcaacagcgc gattgcagct ggagtgtgcg gcgcgctgtt cattggctat
       61 tgcatttact ttgatcgcaa acgccgcagc gatccgaact ttaaatctag attggctact
      121 acacttgaac gtattgagaa gaactttgtc attactgacc caagattgcc agataatccc
      181 attatattcg cgtccgatag tttcttgcag ttgacagaat atagccgtga agaaattttg
      241 ggaagaaact gcaggtttct acaaggtcct gaaactgatc gcgcgacagt gagaaaaatt
      301 agagatgcca tagataacca aacagaggtc actgttcagc tgattaatta tacaaagagt
      361 ggtaaaaagt tctggaacct ctttcacttg cagcctatgc gagatcagaa gggagatgtc
      421 cagtacttta ttggggttca gttggatgga actgagcatg tccgagatgc tgccgagaga
      481 gagggagtca tgctgattaa gaaaactgca gaaaatattg atgaggcggc aaaagaactt
      541 taa
//
