LOCUS       sequence-153398-         995 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Fluorescent Reporter for Dopaminergic Neuron Differentiation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 995)
  AUTHORS   Oh MS, Hong SJ, Huh Y, Kim KS
  TITLE     Expression of transgenes in midbrain dopamine neurons using the 
            tyrosine hydroxylase promoter.
  JOURNAL   Gene Ther. 2009 Mar;16(3):437-40. doi: 10.1038/gt.2008.148. Epub 
            2008 Sep 18.
  PUBMED    18800154
REFERENCE   2  (bases 1 to 995)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/gt.2008.148"; journalName: "Gene Ther"; date: "2009-03"; 
            volume: "16"; issue: "3"; pages: "437-40"
COMMENT     Sequence Label: pAAV2.5-THP-GFP
FEATURES             Location/Qualifiers
     source          1..995
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(join(100..813,814..816,817..819))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(103..813,814..816,817..819))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(145..166)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     494..513
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     753..774
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      816..825
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 cagggccagg agaggcactg gggaggggtc acagggatgc cacccgtaga tctctcgagc
       61 agcgctgctc gaggcaagct tctgcaggtc gactctagat tacttgtaca gctcgtccat
      121 gccgagagtg atcccggcgg cggtcacgaa ctccagcagg accatgtgat cgcgcttctc
      181 gttggggtct ttgctcaggg cggactgggt gctcaggtag tggttgtcgg gcagcagcac
      241 ggggccgtcg ccgatggggg tgttctgctg gtagtggtcg gcgagctgca cgctgccgtc
      301 ctcgatgttg tggcggatct tgaagttcac cttgatgccg ttcttctgct tgtcggccat
      361 gatatagacg ttgtggctgt tgtagttgta ctccagcttg tgccccagga tgttgccgtc
      421 ctccttgaag tcgatgccct tcagctcgat gcggttcacc agggtgtcgc cctcgaactt
      481 cacctcggcg cgggtcttgt agttgccgtc gtccttgaag aagatggtgc gctcctggac
      541 gtagccttcg ggcatggcgg acttgaagaa gtcgtgctgc ttcatgtggt cggggtagcg
      601 gctgaagcac tgcacgccgt aggtcagggt ggtcacgagg gtgggccagg gcacgggcag
      661 cttgccggtg gtgcagatga acttcagggt cagcttgccg taggtggcat cgccctcgcc
      721 ctcgccggac acgctgaact tgtggccgtt tacgtcgccg tccagctcga ccaggatggg
      781 caccaccccg gtgaacagct cctcgccctt gctcaccatg gtggcgtcta gaggatcccc
      841 gggtaccgag ctcgaattcc tctggtggtc ccgagttctg tctccacagc cctcgccagg
      901 caggcgccct ctttaaaggc caggctgacg tcaaagcccc tctgggtccc ccacctccta
      961 cctcctgcgc atcctctcca cgcctgctgt gcctg
//
