LOCUS       sequence-153399-         882 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of Ca2+ sensor GAP in the nucleus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 882)
  AUTHORS   Rodriguez-Garcia A, Rojo-Ruiz J, Navas-Navarro P, Aulestia FJ, 
            Gallego-Sandin S, Garcia-Sancho J, Alonso MT
  TITLE     GAP, an aequorin-based fluorescent indicator for imaging Ca2+ in 
            organelles.
  JOURNAL   Proc Natl Acad Sci U S A. 2014 Feb 18;111(7):2584-9. doi: 
            10.1073/pnas.1316539111. Epub 2014 Feb 5.
  PUBMED    24501126
REFERENCE   2  (bases 1 to 882)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1316539111"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2014-02-18- 18"; volume: "111"; issue: "7"; pages: "2584-9"
COMMENT     Sequence Label: pcDNA3.nucGAP
FEATURES             Location/Qualifiers
     source          1..882
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(732..752)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 ctatagaata gggccctcta gatgcatgct cgagcggccg ccagtgtgat ggatatctgc
       61 agaattctta ggggacagct ccaccgtaga gcttttcgca agcaggatcc atggtgtacc
      121 aaaatcctaa atgttgtctt gtcatctcat caacatcgag ttgtccactt tcatcaatat
      181 cgcacactct gaatgtttcc tcgcaatctt ctgatgattg gatgatacca gcagctttgg
      241 tgtatgcttt ccattcatcc agtgtaatgg ctccattttg atctttgtca acgatatcaa
      301 acaaagcatc accccatata cggatgagcg ttggttcgtt tttggcgtat ttctccaatt
      361 catcagtagc caattttttc catccttcaa tatatgcagg ccaatcagtt tccacaccat
      421 atttcattcc agctcctccg aagaaggctt ctacagcatc tttgtgtcgt ttggcttgct
      481 caggtgttgc tccaaggtta ttgatgacaa tatcagatgc cttgtagacc atctcgtcaa
      541 gagagatttt tccattgtgg ttgacatcaa ggaaattgaa catatgcttg tgtcgtccaa
      601 tccatcttgg gttgtcgaag tctgatgtaa gtttaccggt acccgcggtc ggtgcagtgg
      661 tcggcgtggt tgctggggtc gctgtacccg gtttgtagag ctcatccatg ccatgtgtaa
      721 tcccagcagc agttacaaac tcaagaagga ccatgtggtc acgcttttcg ttgggatctt
      781 tcgaaagggc agattgtgtc gacaggtaat ggttgtctgg taaaaggaca gggccatcgc
      841 caattggagt attttgttga taatggtctg ctagttgaac gg
//
