LOCUS       sequence-153499-        1014 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Spinach2 under a T7 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1014)
  AUTHORS   Wang XC, Wilson SC, Hammond MC
  TITLE     Next-generation RNA-based fluorescent biosensors enable anaerobic 
            detection of cyclic di-GMP.
  JOURNAL   Nucleic Acids Res. 2016 Jul 5. pii: gkw580.
  PUBMED    27382070
REFERENCE   2  (bases 1 to 1014)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2016 Jul 5. pii: gkw580."
COMMENT     Sequence Label: pET31b-T7-Spinach2
FEATURES             Location/Qualifiers
     source          1..1014
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     273..292
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     376..395
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        376..394
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     terminator      564..610
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             617..634
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(687..705)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      701..748
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(872..891)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 tgaattgact ctcttccggg cgctatcatg ccataccgcg aaaggttttg cgccattcga
       61 tggtgtccgg gatctcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt
      121 agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg
      181 cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg
      241 agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca
      301 accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc
      361 tcgatcccgc gaaattaata cgactcacta taggggcccg gatagctcag tcggtagagc
      421 agcggccgga tgtaactgaa tgaaatggtg aaggacgggt ccagtaggct gcttcggcag
      481 cctacttgtt gagtagagtg tgagctccgt aactagttac atccggccgc gggtccaggg
      541 ttcaagtccc tgttcgggcg ccatagcata accccttggg gcctctaaac gggtcttgag
      601 gggttttttg ctcgagcacc accaccacca ccactgagat ccggctgcta acaaagcccg
      661 aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc
      721 ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gattggcgaa
      781 tgggacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg
      841 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc
      901 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga
      961 tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttca
//
