LOCUS       sequence-153504-        1025 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Spinach2 under a T7 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1025)
  AUTHORS   Wang XC, Wilson SC, Hammond MC
  TITLE     Next-generation RNA-based fluorescent biosensors enable anaerobic 
            detection of cyclic di-GMP.
  JOURNAL   Nucleic Acids Res. 2016 Jul 5. pii: gkw580.
  PUBMED    27382070
REFERENCE   2  (bases 1 to 1025)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2016 Jul 5. pii: gkw580."
COMMENT     Sequence Label: pET31b-T7-Spinach2
FEATURES             Location/Qualifiers
     source          1..1025
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(198..302)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(328..783)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(465..486)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     677..696
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     terminator      complement(820..867)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     863..881
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     CDS             complement(934..951)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      complement(958..1004)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
       61 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
      121 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
      181 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
      241 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
      301 cgcacatttc cccgaaaagt gccacctaaa ttgtaagcgt taatattttg ttaaaattcg
      361 cgttaaattt ttgttaaatc agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc
      421 cttataaatc aaaagaatag accgagatag ggttgagtgt tgttccagtt tggaacaaga
      481 gtccactatt aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg
      541 atggcccact acgtgaacca tcaccctaat caagtttttt ggggtcgagg tgccgtaaag
      601 cactaaatcg gaaccctaaa gggagccccc gatttagagc ttgacgggga aagccggcga
      661 acgtggcgag aaaggaaggg aagaaagcga aaggagcggg cgctagggcg ctggcaagtg
      721 tagcggtcac gctgcgcgta accaccacac ccgccgcgct taatgcgccg ctacagggcg
      781 cgtcccattc gccaatccgg atatagttcc tcctttcagc aaaaaacccc tcaagacccg
      841 tttagaggcc ccaaggggtt atgctagtta ttgctcagcg gtggcagcag ccaactcagc
      901 ttcctttcgg gctttgttag cagccggatc tcaatggtgg tggtggtggt gctcgagcaa
      961 aaaacccctc aagacccgtt tagaggcccc aaggggttat gctatggcgc ccgaacaggg
     1021 acttg
//
