LOCUS       sequence-153514-        1023 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Dual expression of Ct biosensor and YhjH under individual T7 
            promoters.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1023)
  AUTHORS   Wang XC, Wilson SC, Hammond MC
  TITLE     Next-generation RNA-based fluorescent biosensors enable anaerobic 
            detection of cyclic di-GMP.
  JOURNAL   Nucleic Acids Res. 2016 Jul 5. pii: gkw580.
  PUBMED    27382070
REFERENCE   2  (bases 1 to 1023)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2016 Jul 5. pii: gkw580."
COMMENT     Sequence Label: pETDuet-T7-Ct-YhjH
FEATURES             Location/Qualifiers
     source          1..1023
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     276..295
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     383..402
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        383..401
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    402..426
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             441..463
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 ccctgaattg actctcttcc gggcgctatc atgccatacc gcgaaaggtt ttgcgccatt
       61 cgatggtgtc cgggatctcg acgctctccc ttatgcgact cctgcattag gaagcagccc
      121 agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg
      181 gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc
      241 atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca
      301 gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag
      361 atcgatctcg atcccgcgaa attaatacga ctcactatag gggaattgtg agcggataac
      421 aattcccctc tagaaataat tttgtttaac tttaagaagg agatatacct atgataaggc
      481 aggttatcca gcgaataagc aaccctgaag caagcatcga gagcttgcag gaacggcgtt
      541 tttggttgca gtgtgagcgt gcttacacct ggcagccgat ctatcaaaca tgcgggcggt
      601 taatggccgt ggagctatta acggtggtca cgcatccctt gaacccttcg caacgcctgc
      661 cgccggatcg ctattttact gaaatcaccg tcagccatcg gatggaggtt gtgaaagagc
      721 agattgattt gctggcgcaa aaagccgact tctttataga gcacggcctg ctggcatcgg
      781 tcaatattga tggccctacg ctcatcgccc tgcgtcagca accaaaaatc ctgcgccaga
      841 ttgagcgtct tccctggctg cgtttcgaac tggtggagca tatccgtctg ccgaaagatt
      901 caacctttgc ctcgatgtgt gaatttggcc cgctgtggct ggatgatttt ggtaccggga
      961 tggcaaattt ctctgcgcta agtgaagtgc gttatgacta catcaaaatc gcgcgagaac
     1021 tgt
//
