LOCUS       sequence-153535-         664 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  recipient plasmid [single nuclease]; pPcUbi:Cas9, BASTA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 664)
  AUTHORS   Ordon J, Gantner J, Kemna J, Schwalgun L, Reschke M, Streubel J, 
            Boch J, Stuttmann J
  TITLE     Generation of chromosomal deletions in dicotyledonous plants 
            employing a user-friendly genome editing toolkit.
  JOURNAL   Plant J. 2016 Aug 31. doi: 10.1111/tpj.13319.
  PUBMED    27579989
REFERENCE   2  (bases 1 to 664)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant J. 
            2016 Aug 31. doi: 10.1111/tpj.13319."
COMMENT     Sequence Label: pDGE65
FEATURES             Location/Qualifiers
     source          1..664
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        129..204
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    518..542
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
ORIGIN
        1 ggtttccgtt atcggggaag aagtggctga tctcagccac cgcgaaaatg acatcaaaaa
       61 cgccattaac ctgatgttct ggggaatata aatgtcaggc tcccttatac acagccagtc
      121 gaagacttgt tttagagcta gaaatagcaa gttaaaataa ggctagtccg ttatcaactt
      181 gaaaaagtgg caccgagtcg gtgctttttt ttgcttccat gctagagtcc gcaaaaatca
      241 ccagtctctc tctacaaatc tatctctctc tatttttctc cagaataatg tgtgagtagt
      301 tcccagataa gggaattagg gttcttatag ggtttcgctc atgtgttgag catataagaa
      361 acccttagta tgtatttgta tttgtaaaat acttctatca ataaaatttc taattcctaa
      421 aaccaaaatc cagtgacctg caggcatgcg acgtcgagct tagcttgagc ttggatcaga
      481 ttgtcgtttc ccgccttcag tttaaactat cagtgtttga caggatatat tggcgggtaa
      541 acctaagaga aaagagcgtt tattagaata atcggatatt taaaagggcg tgaaaaggtt
      601 tatccgttcg tccatttgta tgtgcatgcc aaccacaggg ttcccctcgg gatcaaagta
      661 cttt
//
