LOCUS       sequence-153672-         612 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  All-in-One piggyBac transposon Destination vector for dox-inducible 
            expression of Gateway cloned elements (inducible GFP and 
            constitutive rtTA and puromycin resistance).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 612)
  AUTHORS   Kim SI, Oceguera-Yanez F, Sakurai C, Nakagawa M, Yamanaka S, Woltjen
            K
  TITLE     Inducible Transgene Expression in Human iPS Cells Using Versatile 
            All-in-One piggyBac Transposons.
  JOURNAL   Methods Mol Biol. 2016;1357:111-31. doi: 10.1007/7651_2015_251.
  PUBMED    26025620
REFERENCE   2  (bases 1 to 612)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods Mol
            Biol."; date: "2016"; volume: "1357:111-31. doi"; pages: " 
            10.1007/7651_2015_251"
COMMENT     Sequence Label: PB-TAG-ERP2
FEATURES             Location/Qualifiers
     source          1..612
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    join(23..31,32..34,35..609)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(196..215)
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     425..442
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
ORIGIN
        1 gctcctcgcc cttgctcacc atggttgtgg ccatattatc atcgtgtttt tcaaaggaaa
       61 accacgtccc cgtggttcgg ggggcctaga cgttttttta acctcgacta aacacatgta
      121 aagcatgtgc accgaggccc cagatcagat cccatacaat ggggtacctt ctgggcatcc
      181 ttcagcccct tgttgaatac gcttgaggag agccatttga ctctttccac aactatccaa
      241 ctcacaacgt ggcactgggg ttgtgccgcc tttgcaggtg tatcttatac acgtggcttt
      301 tggccgcaga ggcacctgtc gccaggtggg gggttccgct gcctgcaaag ggtcgctaca
      361 gacgttgttt gtcttcaaga agcttccaga ggaactgctt ccttcacgac attcaacaga
      421 ccttgcattc ctttggcgag aggggaaaga cccctaggaa tgctcgtcaa gaagacaggg
      481 ccaggtttcc gggccctcac attgccaaaa gacggcaata tggtggaaaa taacatatag
      541 acaaacgcac accggcctta ttccaagcgg cttcggccag taacgttagg ggggggggag
      601 ggagaggggg gg
//
