LOCUS       sequence-153688-         844 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  donor vector for AAVS1 targeting (neomycin selection) with multiple 
            cloning site.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 844)
  AUTHORS   Oceguera-Yanez F, Kim SI, Matsumoto T, Tan GW, Xiang L, Hatani T, 
            Kondo T, Ikeya M, Yoshida Y, Inoue H, Woltjen K
  TITLE     Engineering the AAVS1 locus for consistent and scalable transgene 
            expression in human iPSCs and their differentiated derivatives.
  JOURNAL   Methods. 2015 Dec 18. pii: S1046-2023(15)30181-X. doi: 
            10.1016/j.ymeth.2015.12.012.
  PUBMED    26707206
REFERENCE   2  (bases 1 to 844)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods. 
            2015 Dec 18. pii: S1046-2023(15)30181-X. doi: 
            10.1016/j.ymeth.2015.12.012."
COMMENT     Sequence Label: pAAVS1-Nst-MCS
FEATURES             Location/Qualifiers
     source          1..844
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    169..670
                     /label=HA-L
                     /note="left homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
     misc_feature    797..822
                     /label=SA
                     /note="splice acceptor site"
ORIGIN
        1 agcattctct cccctgggcc tgtgccgctt tctgtctgca gcttgtggcc tgggtcacct
       61 ctacggctgg cccagatcct tccctgccgc ctccttcagg ttccgtcttc ctccactccc
      121 tcttcccctt gctctctgct gtgttgctgc ccaaggatgc tctttccgga gcacttcctt
      181 ctcggcgctg caccacgtga tgtcctctga gcggatcctc cccgtgtctg ggtcctctcc
      241 gggcatctct cctccctcac ccaaccccat gccgtcttca ctcgctgggt tcccttttcc
      301 ttctccttct ggggcctgtg ccatctctcg tttcttagga tggccttctc cgacggatgt
      361 ctcccttgcg tcccgcctcc ccttcttgta ggcctgcatc atcaccgttt ttctggacaa
      421 ccccaaagta ccccgtctcc ctggctttag ccacctctcc atcctcttgc tttctttgcc
      481 tggacacccc gttctcctgt ggattcgggt cacctctcac tcctttcatt tgggcagctc
      541 ccctaccccc cttacctctc tagtctgtgc tagctcttcc agccccctgt catggcatct
      601 tccaggggtc cgagagctca gctagtcttc ttcctccaac ccgggcccct atgtccactt
      661 caggacagca tgtttgctgc ctccagggat cctgtgtccc cgagctggga ccaccttata
      721 ttcccagggc cggttaatgt ggctctggtt ctgggtactt ttatctgtcc cctccacccc
      781 acagtggggc aagcttctga cctcttctct tcctcccaca gggcctcgag agatctggca
      841 gcgg
//
