LOCUS       sequence-153690-         841 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  donor vector for AAVS1 targeting (neomycin selection) with multiple 
            cloning site.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 841)
  AUTHORS   Oceguera-Yanez F, Kim SI, Matsumoto T, Tan GW, Xiang L, Hatani T, 
            Kondo T, Ikeya M, Yoshida Y, Inoue H, Woltjen K
  TITLE     Engineering the AAVS1 locus for consistent and scalable transgene 
            expression in human iPSCs and their differentiated derivatives.
  JOURNAL   Methods. 2015 Dec 18. pii: S1046-2023(15)30181-X. doi: 
            10.1016/j.ymeth.2015.12.012.
  PUBMED    26707206
REFERENCE   2  (bases 1 to 841)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods. 
            2015 Dec 18. pii: S1046-2023(15)30181-X. doi: 
            10.1016/j.ymeth.2015.12.012."
COMMENT     Sequence Label: pAAVS1-Nst-MCS
FEATURES             Location/Qualifiers
     source          1..841
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    112..641
                     /label=HA-R
                     /note="right homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
ORIGIN
        1 cctgtaggga aggggcagga gagccagggg catgagatgg tggacgagga agggggacag
       61 ggaagcctga gcgcctctcc tgggcttgcc aaggactcaa acccagaagc ccagagcagg
      121 gccttaggga agcgggaccc tgctctgggc ggaggaatat gtcccagata gcactgggga
      181 ctctttaagg aaagaaggat ggagaaagag aaagggagta gaggcggcca cgacctggtg
      241 aacacctagg acgcaccatt ctcacaaagg gagttttcca cacggacacc cccctcctca
      301 ccacagccct gccaggacgg ggctggctac tggccttatc tcacaggtaa aactgacgca
      361 cggaggaaca atataaattg gggactagaa aggtgaagag ccaaagttag aactcaggac
      421 caacttattc tgattttgtt tttccaaact gcttctcctc ttgggaagtg taaggaagct
      481 gcagcaccag gatcagtgaa acgcaccaga cagccgcgtc agagcagctc aggttctggg
      541 agagggtagc gcagggtggc cactgagaac cgggcaggtc acgcatcccc cccttccctc
      601 ccaccccctg ccaagctctc cctcccagga tcctctctgg ctccatcgta agcaaacctt
      661 agaggttctg gcaaggagag agatggctcc aggaaatggg ggtgtgtcac cagataagga
      721 atctgcctaa caggaggtgg gggttagacc caatatcagg agactaggaa ggaggaggcc
      781 taaggatggg gcttttctgt caaagcttag tactgtcgac ttctagattt aattaatact
      841 a
//
