LOCUS       sequence-153722-         786 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mammalian expression of Zdk1 tagged Rac1 Q61L.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 786)
  AUTHORS   Wang H, Vilela M, Winkler A, Tarnawski M, Schlichting I, Yumerefendi
            H, Kuhlman B, Liu R, Danuser G, Hahn KM
  TITLE     LOVTRAP: an optogenetic system for photoinduced protein 
            dissociation.
  JOURNAL   Nat Methods. 2016 Jul 18. doi: 10.1038/nmeth.3926.
  PUBMED    27427858
REFERENCE   2  (bases 1 to 786)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2016 Jul 18. doi: 10.1038/nmeth.3926."
COMMENT     Sequence Label: pTriEx-mCherry-Zdk1-Rac1 Q61L
FEATURES             Location/Qualifiers
     source          1..786
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..177
                     /codon_start=1
                     /product="""Zdark"" protein, derived from the Z subunit of 
                     protein A, that binds to the dark state of LOV2 and is 
                     released upon irradiation (Wang et al., 2016)"
                     /label=Zdk1
                     /translation="MVDNKFNKEKTRAGAEIHSLPNLNVEQKFAFIVSLFDDPSQSANL
                     LAEAKKLNDAQAPK"
     CDS             join(208..387,388..390,391..786)
                     /codon_start=1
                     /product="constitutively active mutant of human Rac family 
                     small GTPase 1"
                     /label=Rac1 (Q61L)
                     /translation="MQAIKCVVVGDGAVGKTCLLISYTTNAFPGEYIPTVFDNYSANVM
                     VDGKPVNLGLWDTAGLEDYDRLRPLSYPQTDVFLICFSLVSPASFENVRAKWYPEVRHH
                     CPNTPIILVGTKLDLRDDKDTIEKLKEKKLTPITYPQGLAMAKEIGAVKYLECSALTQR
                     GLKTVFDEAIRAVLCPPPVKKRKRKCLLL"
ORIGIN
        1 atggtggata acaaattcaa taaagaaaag acgcgtgccg gtgcggaaat ccattctctg
       61 ccaaacctga atgttgagca gaagtttgcc ttcatcgtga gcctgtttga tgatccatct
      121 cagagcgcca atctgctggc cgaagccaaa aaactgaacg atgcccaggc cccaaaagga
      181 tccggtggtt ctggttctgg tggtaccatg caggccatca agtgtgtggt ggtgggagac
      241 ggagctgtag gtaaaacttg cctactgatc agttacacaa ccaatgcatt tcctggagaa
      301 tatatcccta ctgtctttga caattattct gccaatgtta tggtagatgg aaaaccggtg
      361 aatctgggct tatgggatac agctggacta gaagattatg acagattacg ccccctatcc
      421 tatccgcaaa cagatgtgtt cttaatttgc ttttcccttg tgagtcctgc atcatttgaa
      481 aatgtccgtg caaagtggta tcctgaggtg cggcaccact gtcccaacac tcccatcatc
      541 ctagtgggaa ctaaacttga tcttagggat gataaagaca cgatcgagaa actgaaggag
      601 aagaagctga ctcccatcac ctatccgcag ggtctagcca tggctaagga gattggtgcc
      661 gtaaaatacc tggagtgctc ggcgctcaca cagcgaggcc tcaagacagt gtttgacgaa
      721 gcgatccgag cagtcctctg cccgcctccc gtgaagaaga ggaagagaaa atgcctgctg
      781 ttgtaa
//
