LOCUS       sequence-153758-         718 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPR/Cas9 DROSHA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 718)
  AUTHORS   Cirera-Salinas D, Yu J, Bodak M, Ngondo RP, Herbert KM, Ciaudo C
  TITLE     Noncanonical function of DGCR8 controls mESC exit from pluripotency.
  JOURNAL   J Cell Biol. 2017 Jan 18. pii: jcb.201606073. doi: 
            10.1083/jcb.201606073.
  PUBMED    28100686
REFERENCE   2  (bases 1 to 718)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol. 2017 Jan 18. pii: jcb.201606073. doi: 10.1083/jcb.201606073."
COMMENT     Sequence Label: pX330-sgRNA_Drosha_2
FEATURES             Location/Qualifiers
     source          1..718
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     117..136
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        216..291
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     enhancer        388..673
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer; 
                     contains an 18-bp deletion relative to the standard CMV 
                     enhancer"
ORIGIN
        1 gagagataat tggaattaat ttgactgtaa acacaaagat attagtacaa aatacgtgac
       61 gtagaaagta ataatttctt gggtagtttg cagttttaaa attatgtttt aaaatggact
      121 atcatatgct taccgtaact tgaaagtatt tcgatttctt ggctttatat atcttgtgga
      181 aaggacgaaa caccggcggc ttcctgtcca tgacagtttt agagctagaa atagcaagtt
      241 aaaataaggc tagtccgtta tcaacttgaa aaagtggcac cgagtcggtg cttttttgtt
      301 ttagagctag aaatagcaag ttaaaataag gctagtccgt ttttagcgcg tgcgccaatt
      361 ctgcagacaa atggctctag aggtacccgt tacataactt acggtaaatg gcccgcctgg
      421 ctgaccgccc aacgaccccc gcccattgac gtcaatagta acgccaatag ggactttcca
      481 ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta
      541 tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcattg
      601 tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat
      661 cgctattacc atggtcgagg tgagccccac gttctgcttc actctcccca tctccccc
//
