LOCUS       sequence-153760-         756 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 756)
  AUTHORS   Edie S, Zaghloul NA, Leitch CC, Klinedinst DK, Lebron J, Thole JF, 
            McCallion AS, Katsanis N, Reeves RH
  TITLE     Survey of Human Chromosome 21 Gene Expression Effects on Early 
            Development in Danio rerio.
  JOURNAL   G3 (Bethesda). 2018 Jul 2;8(7):2215-2223. doi: 
            10.1534/g3.118.200144.
  PUBMED    29760202
REFERENCE   2  (bases 1 to 756)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1534/g3.118"; 
            journalName: "G3 (Bethesda)"; date: "2018-07-2- 2"; volume: "8"; 
            issue: "7"; pages: "2215-2223"
COMMENT     Sequence Label: TPTE_pCSdest
FEATURES             Location/Qualifiers
     source          1..756
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        37..55
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     37..54
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     protein_bind    109..133
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 accgccattc tgcctgggga cgtcggagca agcttgattt aggtgacact atagaataca
       61 agctacttgt tctttttgca ggatcccatc gattcgaatt caaggatcac aagtttgtac
      121 aaaaaagcag gcaccatgaa tgaaagtcct gatccgactg acctggcggg agtcatcatt
      181 gagctcggcc ccaatgacag tccacagaca agtgaattta aaggagcaac cgaggaggca
      241 cctgcgaaag aaagtgtgtt agcacgactt tccaagtttg aagttgaaga tgctgaaaat
      301 gttgcttcat atgacagcaa gattaagaaa attgtgcatt caattgtatc atcctttgca
      361 tttggactat ttggagtttt cctggtctta ctggatgtca ctctcatcct tgccgaccta
      421 attttcactg acagcaaact ttatattcct ttggagtatc gttctatttc tctagctatt
      481 gccttatttt ttctcatgga tgttcttctt cgagtatttg tagaaaggag acagcagtat
      541 ttttctgact tatttaacat tttagatact gccattattg tgattcttct gctggttgat
      601 gtcgtttaca ttttttttga cattaagttg cttaggaata ttcccagatg gacacattta
      661 cttcgacttc tacgacttat tattctgtta agaatttttc atctgtttca tcaaaaaaga
      721 caacttgaaa agctgataag aaggcgggtt tcagaa
//
