LOCUS       sequence-153776-         748 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 748)
  AUTHORS   Edie S, Zaghloul NA, Leitch CC, Klinedinst DK, Lebron J, Thole JF, 
            McCallion AS, Katsanis N, Reeves RH
  TITLE     Survey of Human Chromosome 21 Gene Expression Effects on Early 
            Development in Danio rerio.
  JOURNAL   G3 (Bethesda). 2018 Jul 2;8(7):2215-2223. doi: 
            10.1534/g3.118.200144.
  PUBMED    29760202
REFERENCE   2  (bases 1 to 748)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1534/g3.118"; 
            journalName: "G3 (Bethesda)"; date: "2018-07-2- 2"; volume: "8"; 
            issue: "7"; pages: "2215-2223"
COMMENT     Sequence Label: OLIG2_pCSdest
FEATURES             Location/Qualifiers
     source          1..748
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        37..55
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     37..54
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     protein_bind    109..133
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 accgccattc tgcctgggga cgtcggagca agcttgattt aggtgacact atagaataca
       61 agctacttgt tctttttgca ggatcccatc gattcgaatt caaggatcac aagtttgtac
      121 aaaaaagcag gcaccatgga ctcggacgcc agcctggtgt ccagccgccc gtcgtcgcca
      181 gagcccgatg acctttttct gccggcccgg agtaagggca gcagcggcag cgccttcact
      241 gggggcaccg tgtcctcgtc caccccgagt gactgcccgc cggagctgag cgccgagctg
      301 cgcggcgcta tgggctctgc gggcgcgcat cctgtggaca agctaggagg cagtggcttc
      361 aagtcatcct cgtccagcac ctcgtcgtct acgtcgtcgg cggctgcgtc gtccaccaag
      421 aaggacaaga agcaaatgac agagccggag ctgcagcagc tgcgtctcaa gatcaacagc
      481 cgcgagcgca agcgcatgca cgacctcaac atcgccatgg atggcctccg cgaggtcatg
      541 ccgtacgcac acggcccttc ggtgcgcaag ctttccaaga tcgccacgct gctgctggcg
      601 cgcaactaca tcctcatgct caccaactcg ctggaggaga tgaagcgact ggtgagcgag
      661 atctacgggg gccaccacgc tggcttccac ccgtcggcct gcggcggcct ggcgcactcc
      721 gcgcccctgc ccgccgccac cgcgcacc
//
