LOCUS       sequence-153788-         726 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 726)
  AUTHORS   Edie S, Zaghloul NA, Leitch CC, Klinedinst DK, Lebron J, Thole JF, 
            McCallion AS, Katsanis N, Reeves RH
  TITLE     Survey of Human Chromosome 21 Gene Expression Effects on Early 
            Development in Danio rerio.
  JOURNAL   G3 (Bethesda). 2018 Jul 2;8(7):2215-2223. doi: 
            10.1534/g3.118.200144.
  PUBMED    29760202
REFERENCE   2  (bases 1 to 726)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1534/g3.118"; 
            journalName: "G3 (Bethesda)"; date: "2018-07-2- 2"; volume: "8"; 
            issue: "7"; pages: "2215-2223"
COMMENT     Sequence Label: IGSF5_pCSdest
FEATURES             Location/Qualifiers
     source          1..726
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        37..55
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     37..54
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     protein_bind    109..133
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 accgccattc tgcctgggga cgtcggagca agcttgattt aggtgacact atagaataca
       61 agctacttgt tctttttgca ggatcccatc gattcgaatt caaggatcac aagtttgtac
      121 aaaaaagcag gcaccatggg tcagaaggaa aggagcacag cagatactct gccggatctg
      181 gaggaatgga agtcagcggc gggtctgcga tggtggcaaa cagcagtggt ggacggttct
      241 gggtctggta atgaagtcat agaaggcccc caaaatgcaa gagtcctgaa gggctcccag
      301 gctcgcttca actgcaccgt ctcccagggc tggaagctca tcatgtgggc tctcagtgac
      361 atggtggtgc taagcgtcag gcccatggag cccatcatca ccaatgaccg cttcacctct
      421 cagaggtacg accagggcgg gaacttcacc tcggagatga tcatccacaa tgtggagccc
      481 agtgattcgg ggaacatcag atgcagcctc cagaacagtc gcctgcatgg atctgcttac
      541 cttaccgtcc aagttatggg agagctgttc attcccagtg ttaatcttgt agtcgctgag
      601 aatgaacctt gtgaagttac ttgtctaccc tcacactgga cccggctccc ggatatttcc
      661 tgggagctcg gtctcctggt cagccattca agctattatt ttgttccgga gcccagcgac
      721 cttcaa
//
