LOCUS       sequence-153887-         828 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 828)
  AUTHORS   Edie S, Zaghloul NA, Leitch CC, Klinedinst DK, Lebron J, Thole JF, 
            McCallion AS, Katsanis N, Reeves RH
  TITLE     Survey of Human Chromosome 21 Gene Expression Effects on Early 
            Development in Danio rerio.
  JOURNAL   G3 (Bethesda). 2018 Jul 2;8(7):2215-2223. doi: 
            10.1534/g3.118.200144.
  PUBMED    29760202
REFERENCE   2  (bases 1 to 828)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1534/g3.118"; 
            journalName: "G3 (Bethesda)"; date: "2018-07-2- 2"; volume: "8"; 
            issue: "7"; pages: "2215-2223"
COMMENT     Sequence Label: C21orf37_pCSdest
FEATURES             Location/Qualifiers
     source          1..828
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    37..61
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(505..529)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        complement(583..601)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(584..601)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 tacgactcac tatagttcta gaggctcgag aggatcacca ctttgtacaa gaaagctggg
       61 ttcccttgag catgttgcat gatgtctgcc ttctccgtca ctctgtaatt tctctctttt
      121 tctctctctc atcttctgta gtctcaacta cccagaaggc tgaggcagga ggattagttg
      181 agtccaggag atcgaggctg cagtgagctg tgattgcacc actgtactcc agcctaggtg
      241 acagaataag actcctttgt tctagagacc tttcaaagga cagaattcct tttctcaagc
      301 attgagctct ctccatcttt agagtctttt cagtctgttt cacacagtgt cagaagaagg
      361 ttgcctcaaa agttggtaga attactgtaa ttccctccat gacagtagta aagttggatc
      421 ctgctggtag atccggaatg aaatgcagct ggaggatgga gcagattgga ggtgggaaca
      481 gggaacactc agcaggctcc caggtgcctg cttttttgta caaacttgtg atccttgaat
      541 tcgaatcgat gggatcctgc aaaaagaaca agtagcttgt attctatagt gtcacctaaa
      601 tcaagcttgc tccgacgtcc ccaggcagaa tggcggttcc ctaaacgagc attgcttata
      661 tagacctccc attaggcacg cctaccgccc atttacgtca atggaacgcc catttgcgtc
      721 attgcccctc cccattgacg tcaatgggga tgtacttggc agccatcgcg ggccatttac
      781 cgccattgac gtcaatggga gtactgccaa tgtaccctgg cgtacttc
//
